Induction of Golli-MBP expression in CNS macrophages during acute LPS-induced CNS inflammation and experimental autoimmune encephalomyelitis (EAE)

Autor: Patricia E. Danielson, Caroline C. Whitacre, J. Gregor Sutcliffe, Monica J. Carson, J. Cameron Thrash, Tracey L. Papenfuss, Brian S. Hllbrush, Pamela E. Foye
Rok vydání: 2007
Předmět:
Central Nervous System
Lipopolysaccharides
Messenger
lcsh:Medicine
microglia
Neurodegenerative
Inbred C57BL
lcsh:Technology
neuroinflammation
Mice
Technical Report
Macrophage
2.1 Biological and endogenous factors
Aetiology
lcsh:Science
Encephalomyelitis
Cells
Cultured
General Environmental Science
Cultured
biology
Microglia
Experimental autoimmune encephalomyelitis
Neurodegeneration
autoimmunity
General Medicine
medicine.anatomical_structure
Acute Disease
medicine.symptom
Multiple Sclerosis
Encephalomyelitis
Autoimmune
Experimental
General Science & Technology
Cells
Central nervous system
Inflammation
Nerve Tissue Proteins
In situ hybridization
Autoimmune Disease
General Biochemistry
Genetics and Molecular Biology
Experimental
Interferon-gamma
medicine
Animals
RNA
Messenger
lcsh:T
Macrophages
lcsh:R
Neurosciences
Myelin Basic Protein
medicine.disease
Myelin basic protein
Brain Disorders
Mice
Inbred C57BL
Gene Expression Regulation
Immunology
biology.protein
RNA
lcsh:Q
Autoimmune
Transcription Factors
Zdroj: The Scientific World Journal
TheScientificWorldJournal, vol 7, iss SUPPL. 2
The Scientific World Journal, Vol 7, Pp 112-120 (2007)
ISSN: 1537-744X
Popis: Microglia are the tissue macrophages of the CNS. Microglial activation coupled with macrophage infiltration is a common feature of many classic neurodegenerative disorders. The absence of cell-type specific markers has confounded and complicated the analysis of cell-type specific contributions toward the onset, progression, and remission of neurodegeneration. Molecular screens comparing gene expression in cultured microglia and macrophages identified Golli-myelin basic protein (MBP) as a candidate molecule enriched in peripheral macrophages.In situhybridization analysis of LPS/IFNg and experimental autoimmune encephalomyelitis (EAE)–induced CNS inflammation revealed that only a subset of CNS macrophages express Golli-MBP. Interestingly, the location and morphology of Golli-MBP+ CNS macrophages differs between these two models of CNS inflammation. These data demonstrate the difficulties of extendingin vitroobservations toin vivobiology and concretely illustrate the complex heterogeneity of macrophage activation states present in region- and stage-specific phases of CNS inflammation. Taken altogether, these are consistent with the emerging picture that the phenotype of CNS macrophages is actively defined by their molecular interactions with the CNS microenvironment.
Databáze: OpenAIRE
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