A Comparison of Porphyrin Photosensitizers in Photodynamic Inactivation of RNA and DNA Bacteriophages
| Autor: | Brooke K. Mayer, Matthew A. Bork, Troy Skwor, Joe Heffron |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Porphyrins 030106 microbiology lcsh:QR1-502 virus lcsh:Microbiology Virus Article singlet oxygen Bacteriophage 03 medical and health sciences chemistry.chemical_compound Virology Bacteriophages metalloporphyrin chemistry.chemical_classification Reactive oxygen species Aqueous solution Photosensitizing Agents biology Singlet oxygen RNA biology.organism_classification Porphyrin Combinatorial chemistry blue light Disinfection 030104 developmental biology Infectious Diseases chemistry TMPyP Virus Inactivation DNA |
| Zdroj: | Viruses Volume 13 Issue 3 Viruses, Vol 13, Iss 530, p 530 (2021) |
| ISSN: | 1999-4915 |
| Popis: | Effective broad-spectrum antiviral treatments are in dire need as disinfectants and therapeuticalternatives. One such method of disinfection is photodynamic inactivation, which involvesthe production of reactive oxygen species from dissolved oxygen in response to lightstimulatedphotosensitizers. This study evaluated the efficacy of functionalized porphyrin compoundsfor photodynamic inactivation of bacteriophages as human virus surrogates. A blue-lightlight emitting diode (LED) lamp was used to activate porphyrin compounds in aqueous solution(phosphate buffer). The DNA bacteriophages FX174 and P22 were more resistant to porphyrinTMPyP photodynamic inactivation than RNA bacteriophage fr, with increasing rates of inactivationin the order: FX174 < < P22 < fr. Bacteriophage FX174 was therefore considered a resistant virussuitable for the evaluation of three additional porphyrins. These porphyrins were synthesizedfrom TMPyP by inclusion of a central palladium ion (PdT4) and/or the addition of a hydrophobicC14 chain (PdC14 or C14). While the inactivation rate of bacteriophage FX174 via TMPyP wassimilar to previous reports of resistant viruses, FX174 inactivation increased by a factor of approximately2.5 using the metalloporphyrins PdT4 and PdC14. The order of porphyrin effectiveness wasTMPyP < C14 < PdT4 < PdC14, indicating that both Pd2+ ligation and C14 functionalization aidedvirus inactivation. |
| Databáze: | OpenAIRE |
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