Quantification of IgM molecular response by droplet digital PCR as a potential tool for the early diagnosis of sepsis
| Autor: | José María Eiros, Esther Gómez-Sánchez, Lucia Rico, Ana Rodriguez-Fernandez, Jose Ignacio Gómez Herreras, Verónica Iglesias, Jesus F. Bermejo-Martin, John Wain, Elena Carrasco, Eduardo Tamayo, David Andaluz-Ojeda, María Heredia, Susana Soria, Raquel Almansa, Ana Ávila-Alonso, Ángel Martínez-Martínez |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Male
medicine.medical_specialty Letter Respuesta molecular medicine.medical_treatment Molecular Response Critical Care and Intensive Care Medicine Polymerase Chain Reaction Gastroenterology Systemic inflammatory response Sepsis Infección abdominal Internal medicine medicine Humans Abdominal Infection Aged biology Septic shock business.industry Respiratory infection Immunosuppression medicine.disease Systemic inflammatory response syndrome Early Diagnosis Immunoglobulin M Respuesta inflamatoria sistémica Immunology biology.protein Biomarker (medicine) Female business Abdominal surgery |
| Zdroj: | UVaDOC. Repositorio Documental de la Universidad de Valladolid instname Critical Care |
| ISSN: | 1364-8535 |
| DOI: | 10.1186/cc13910 |
| Popis: | Producción Científica Evaluation of host immune response to infection at the molecular level is a promising avenue to obtain diagnostic and prognostic tools for the clinical management of patients with sepsis. A recent report from Cajander and colleagues [1] has shown the potential of HLA-DR mRNA quantification by real-time PCR as a biomarker of immunosuppression in these patients. IgM is the first immunoglobulin produced in response to infection. In a pilot study, we have employed a next generation quantitative PCR method (nanoliter-sized droplet technology paired with digital PCR (ddPCR)) for detecting the early transcriptomic response of IgM in blood from patients with sepsis. Approval for the study protocol for both scientific and ethical aspects was obtained from the Committee for Clinical Research of Hospital Clínico Universitario, Valladolid, Spain. Written informed consent was obtained directly from each patient or a legal surrogate. The target gene transcript was IGHM, which encodes the constant region of the mu heavy chain, which defines the IgM isotype [2]. In blood, the cells producing IgM transcripts are B lymphocytes expressing CD20 [3], which was employed as housekeeping gene. Instituto de Salud Carlos III (grant PI13/02110) |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink