The genome sequence and transmission of an iflavirus from the brown planthopper, Nilaparvata lugens
| Autor: | Tetsuya Kobayashi, Ritsuko Murakami, Yoshitaka Suetsugu, Nobuhiko Nakashima |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Cancer Research
Molecular Sequence Data Genome Viral Biology Genome Hemiptera Open Reading Frames Viral Proteins symbols.namesake chemistry.chemical_compound Virology RNA polymerase Animals RNA Viruses Whole genome sequencing Sanger sequencing Genetics Contig RNA Sequence Analysis DNA biology.organism_classification Infectious Diseases chemistry Iflaviridae symbols RNA Viral Brown planthopper |
| Zdroj: | Virus Research. 176:179-187 |
| ISSN: | 0168-1702 |
| DOI: | 10.1016/j.virusres.2013.06.005 |
| Popis: | A previously unknown iflavirus has been identified in a laboratory colony of the brown planthopper, Nilaparvata lugens. The iflavirus-like sequence was first identified in contig sequences obtained from transcriptome sequencing (RNA-seq) of the brown planthopper. The complete viral genome was resequenced using the Sanger method. The positive-strand RNA genome was 10,937 nucleotides excluding the 3' poly(A) tail, and contained a single large open reading frame encoding coat proteins in the 5' region and replicases in the 3' region. Conserved motifs for coat proteins, helicase, cysteine protease, and RNA-dependent RNA polymerase were identified in the deduced amino sequence, and the estimated molecular mass of the large polyprotein was 358.6kDa. RT-PCR detection of the viral genome indicated that viral shedding occurred through the honeydews of insects in the infected colony. To test transmission, the collected honeydews were used to feed insects in a non-viruliferous colony. After 7 days, the expected RT-PCR fragment was detected in the insects, indicating that the virus can be transmitted horizontally. This is the first iflavirus identified in planthoppers; thus, we propose the name of the virus as Nilaparvata lugens honeydew virus-1. |
| Databáze: | OpenAIRE |
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