Development of a radioimmunoassay for SR 31747A, a new sigma ligand, in human plasma
| Autor: | G. Pastor, D. Dussossoy, P. Casellas, S. Belugou, J. Combalbert |
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| Rok vydání: | 1996 |
| Předmět: |
Analyte
Chromatography Gas medicine.drug_class T-Lymphocytes Metabolite Clinical Biochemistry Radioimmunoassay Pharmaceutical Science Cross Reactions Monoclonal antibody Binding Competitive Analytical Chemistry Mice chemistry.chemical_compound Cyclohexanes Drug Discovery medicine Animals Humans Receptors sigma Peptide bond Bovine serum albumin Spectroscopy Aniline Compounds Chromatography biology Chemistry Anti-Inflammatory Agents Non-Steroidal Antibodies Monoclonal Reproducibility of Results Succinates Ligand (biochemistry) Microsomes Liver biology.protein Quantitative analysis (chemistry) Spleen |
| Zdroj: | Journal of Pharmaceutical and Biomedical Analysis. 14:783-791 |
| ISSN: | 0731-7085 |
| DOI: | 10.1016/0731-7085(96)01751-7 |
| Popis: | A specific and sensitive radioimmunoassay was developed for SR 31747A, a new sigma ligand, using a monoclonal antibody. This antibody was produced from spleen lymphocytes of a mouse immunized with SR 31747A coupled to bovine serum albumin via a peptide bond using SR 120684A, a succinamic acid derivative of SR 31747A. Negligible binding occurred when metabolites, obtained by chemical synthesis or by “indashvitro” incubation with hepatic microsomal fraction, were tested for cross-reactivity. A quantitative recovery in serum of the exogenous analyte was obtained for all the concentrations tested and the quantification limit was found to be 0.25 ng ml−1 of SR 31747 (the nondashsalified derivative). Intra- and inter-assay relative standard deviations ranged from 6.3–10.9 and from 5.3% to 15.4%, respectively. Furthermore, comparison of results from samples which were assayed by radioimmunoassay and gas chromatography demonstrated an excellent correlation (r = 0.984). |
| Databáze: | OpenAIRE |
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