Isocratic reversed-phase high-performance liquid chromatographic separation of tetracyclines and flumequine controlled by a chaotropic effect
| Autor: | Irena M. Choma, K. Pilorz |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Potassium perchlorate
Resolution (mass spectrometry) Chemical Phenomena Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound medicine Chelation Chromatography High Pressure Liquid Antibacterial agent Chromatography Perchlorates Chemistry Physical Organic Chemistry General Medicine Reversed-phase chromatography Hydrogen-Ion Concentration Sodium Compounds Anti-Bacterial Agents Chaotropic agent chemistry Tetracyclines Flumequine Indicators and Reagents Quinolizines medicine.drug Fluoroquinolones |
| Zdroj: | Journal of chromatography. A. 1031(1-2) |
| ISSN: | 0021-9673 |
| Popis: | Fast isocratic reversed-phase high-performance liquid charomatographic (RP-HPLC) separation of four tetracyclines and flumequine was obtained using a Zorbax SB-C 18 column. Baseline resolution was achieved in 11 min. The peaks were narrow, well separated and without any tails although there was no chelating agents added to the mobile phase. Due to the chaotropic effect, the addition of potassium perchlorate allowed controlling the tetracyclines retention while the retention of flumequine was almost constant. |
| Databáze: | OpenAIRE |
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