Characterization and Expression Analysis of Common BeanHistone Deacetylase 6during Development and Cold Stress Response
| Autor: | Venu Kalavacharla, Kalpalatha Melmaiee, Mayavan Subramani, Adrianne Brown, Rita K. Hayford, Ayalew Ligaba-Osena, Khwaja Hossain |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Article Subject lcsh:QH426-470 Pharmaceutical Science Biology medicine.disease_cause Biochemistry Homology (biology) law.invention 03 medical and health sciences Downregulation and upregulation law Genetics medicine Molecular Biology Escherichia coli Gene 2. Zero hunger Abiotic stress food and beverages HDAC6 lcsh:Genetics 030104 developmental biology Histone biology.protein Recombinant DNA |
| Zdroj: | International Journal of Genomics, Vol 2017 (2017) |
| ISSN: | 2314-4378 2314-436X |
| DOI: | 10.1155/2017/2502691 |
| Popis: | Histone deacetylases (HDACs) are important regulators of gene transcription thus controlling multiple cellular processes. Despite its essential role in plants,HDA6is yet to be validated in common bean. In this study, we show thatHDA6is involved in plant development and stress response. Differential expression ofHDA6was determined in various tissues and the expression was seen to be upregulated with plant age (seedling < flowering < maturity). Higher expression was observed in flowers and pods than in stem, leaf, and root. Upregulation ofHDA6gene during cold stress implies its prominent role in abiotic stress. Furthermore, theHDA6gene was isolated from three common bean genotypes and sequence analyses revealed homology with functionally characterized homologs in model species. The 53 kDa translated product was detected using anHDA6specific antibody and recombinant protein overexpressed inEscherichia colishowed HDAC activityin vitro. To our knowledge, this is the first report in the agriculturally important crop common bean describing the functional characterization and biological role ofHDA6. |
| Databáze: | OpenAIRE |
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