pRb-expressing adenovirus Ad5-Rb attenuates the p53-induced apoptosis in cervical cancer cell lines
| Autor: | Wsb Yeung, Hextan Ys Ngan, T.-G. Huang, Sin-Ming Ip |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Cancer Research
Tumor suppressor gene viruses Genetic Vectors Population Uterine Cervical Neoplasms Apoptosis Transfection medicine.disease_cause Retinoblastoma Protein Adenoviridae HeLa Gene product Tumor Cells Cultured medicine Humans education Osteosarcoma education.field_of_study biology Retinoblastoma protein DNA Neoplasm Flow Cytometry Genes p53 biology.organism_classification Virology Molecular biology Oncology biology.protein Female Tumor Suppressor Protein p53 Carcinogenesis Cell Division |
| Zdroj: | European Journal of Cancer. 37:2475-2483 |
| ISSN: | 0959-8049 |
| DOI: | 10.1016/s0959-8049(01)00308-2 |
| Popis: | The retinoblastoma protein (pRb), the gene product of the first reported tumour suppressor gene, is functionally inactivated by the E7 protein of high-risk human papillomavirus (HPV) found in most human cervical cancers. We have, in this study, constructed an adenoviral vector expressing wild-type pRb (Ad5-Rb) and used the constructed Ad5-Rb to transfect the osteosarcoma cell line Saos-2, and three cervical cancer cell lines HeLa, SiHa and C-33A. Our results showed that pRb caused G1 arrest in Saos-2 cells after transfection with Ad5-Rb. The number of colonies formed by the Ad5-Rb-transfected Saos-2 cells in soft agar was also found to be significantly lower (P |
| Databáze: | OpenAIRE |
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