Decreased Neurofilament L Chain Levels in Cerebrospinal Fluid and Tolerogenic Plasmacytoid Dendritic Cells in Natalizumab-Treated Multiple Sclerosis Patients - Brief Research Report
| Autor: | Adriel S. Moraes, Vinicius O. Boldrini, Alliny C. Dionete, Marilia D. Andrade, Ana Leda F. Longhini, Irene Santos, Amanda D. R. Lima, Veronica A. P. G. Silva, Rafael P. C. Dias Carneiro, Raphael P. S. Quintiliano, Breno B. Ferrari, Alfredo Damasceno, Fernando Pradella, Alessandro S. Farias, Charles P. Tilbery, Renan B. Domingues, Carlos Senne, Gustavo B. P. Fernandes, Felipe von Glehn, Carlos Otavio Brandão, Carla R. A. V. Stella, Leonilda M. B. Santos |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
PD-L1 HLA-G C-C chemokine receptor type 7 Neurosciences. Biological psychiatry. Neuropsychiatry cerebrospinal fluid Flow cytometry neuroinflammation 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Cerebrospinal fluid Natalizumab parasitic diseases medicine HLA-DR Neuroinflammation biology medicine.diagnostic_test business.industry Multiple sclerosis Brief Research Report medicine.disease 030104 developmental biology neurofilament light chain Immunology biology.protein business 030217 neurology & neurosurgery medicine.drug RC321-571 Neuroscience |
| Zdroj: | Frontiers in Cellular Neuroscience Frontiers in Cellular Neuroscience, Vol 15 (2021) |
| ISSN: | 1662-5102 |
| Popis: | BackgroundNeurofilament Light (NfL) chain levels in both cerebrospinal fluid (CSF) and serum have been correlated with the reduction of axonal damage in multiple sclerosis (MS) patients treated with Natalizumab (NTZ). However, little is known about the function of plasmacytoid cells in NTZ-treated MS patients.ObjectiveTo evaluate CSF NfL, serum levels of soluble-HLA-G (sHLA-G), and eventual tolerogenic behavior of plasmacytoid dendritic cells (pDCs) in MS patients during NTZ treatment.MethodsCSF NfL and serum sHLA-G levels were measured using an ELISA assay, while pDCs (BDCA-2+) were accessed through flow cytometry analyses.ResultsCSF levels of NfL were significantly reduced during NTZ treatment, while the serum levels of sHLA-G were increased. Moreover, NTZ treatment enhanced tolerogenic (HLA-G+, CD274+, and HLA-DR+) molecules and migratory (CCR7+) functions of pDCs in the peripheral blood.ConclusionThese findings suggest that NTZ stimulates the production of molecules with immunoregulatory function such as HLA-G and CD274 programmed death-ligand 1 (PD-L1) which may contribute to the reduction of axonal damage represented by the decrease of NfL levels in patients with MS. |
| Databáze: | OpenAIRE |
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