Identification of Immunodominant CD4-Restricted Epitopes Co-Located with Antibody Binding Sites in Individuals Vaccinated with ALVAC-HIV and AIDSVAX B/E
| Autor: | Sorachai Nitayaphan, Nelson L. Michael, John Sidney, Morgane Rolland, Jaranit Kaewkungwal, Jeffrey R. Currier, Anais Valencia-Micolta, Merlin L. Robb, Jerome H. Kim, Mark de Souza, Robert J. O'Connell, Nicos Karasavvas, Silvia Ratto-Kim, Supachai Rerks-Ngarm, Punnee Pitisuttuthum, Alessandro Sette, Mary A. Marovich, Sirinan Madnote |
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| Rok vydání: | 2015 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male Adolescent T cell Human immunodeficiency virus (HIV) lcsh:Medicine HIV Antibodies Biology medicine.disease_cause Epitope Cell Line Young Adult medicine Humans Binding site lcsh:Science AIDS Vaccines Multidisciplinary Linear epitope Immunodominant Epitopes lcsh:R Antigen binding Virology Molecular biology Epitope mapping medicine.anatomical_structure HIV-1 biology.protein lcsh:Q Female Binding Sites Antibody Antibody Epitope Mapping Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 10, Iss 2, p e0115582 (2015) |
| ISSN: | 1932-6203 |
| Popis: | We performed fine epitope mapping of the CD4+ responses in the ALVAC-HIV-AIDSVAX B/E prime-boost regimen in the Thai Phase III trial (RV144). Non-transformed Env-specific T cell lines established from RV144 vaccinees were used to determine the fine epitope mapping of the V2 and C1 responses and the HLA class II restriction. Data showed that there are two CD4+ epitopes contained within the V2 loop: one encompassing the α4β7 integrin binding site (AA179-181) and the other nested between two previously described genetic sieve signatures (AA169, AA181). There was no correlation between the frequencies of CD4+ fine epitope responses and binding antibody. |
| Databáze: | OpenAIRE |
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