Assessment by fluorescence-activated cell sorting of whether sperm-associated immunoglobulin (Ig)G and IgA occur on the same sperm population
| Autor: | Osmond J. D’Cruz, Gilbert G. Haas, Lawrence E. DeBault |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Male
endocrine system Immunocytochemistry Population Fluorescent Antibody Technique Semen Cell Separation Immunofluorescence Immunoglobulin E Immunoenzyme Techniques Iodine Radioisotopes Epitopes Antigen medicine Humans education Infertility Male reproductive and urinary physiology Fluorescent Dyes education.field_of_study medicine.diagnostic_test biology urogenital system Obstetrics and Gynecology Phycoerythrin Flow Cytometry Fluoresceins Spermatozoa Sperm Molecular biology Immunoglobulin A Reproductive Medicine Immunoglobulin G biology.protein Antibody Fluorescein-5-isothiocyanate Thiocyanates |
| Zdroj: | Fertility and Sterility. 54:127-132 |
| ISSN: | 0015-0282 |
| DOI: | 10.1016/s0015-0282(16)53648-6 |
| Popis: | Two-color fluorescence-activated cell sorting of antisperm antibody-positive sperm was used to detect simultaneously the presence of immunoglobulin (Ig)A and IgG antisperm antibodies associated in vivo on a man's sperm. Sperm positive for sperm-associated Ig were analyzed using phycoerythrin-conjugated antihuman IgA and fluorescein isothiocyanate-conjugated antihuman IgG; up to 87% of the same spermatozoa were stained with both labels. Sperm positive for only one of the antisperm antibody isotypes stained up to 90% of a man's sperm with only one fluorochrome. Immunocytochemistry studies revealed similar patterns of sperm binding for sperm-associated IgG and IgA. These results suggest that the sperm antigenic determinants reacting with antisperm IgA and IgG are present on the same sperm population at similar locations on the sperm surface. |
| Databáze: | OpenAIRE |
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