Cross-feeding between Bifidobacterium infantis and Anaerostipes caccae on lactose and human milk oligosaccharides
| Autor: | Chia, L W, Mank, M, Blijenberg, B, Bongers, R S, van Limpt, K, Wopereis, H, Tims, S, Stahl, B, Belzer, C, Knol, J, Pharmacology, Afd Pharmacology, Afd Chemical Biology and Drug Discovery, Chemical Biology and Drug Discovery |
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| Přispěvatelé: | Pharmacology, Afd Pharmacology, Afd Chemical Biology and Drug Discovery, Chemical Biology and Drug Discovery |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Microbiology (medical) 030106 microbiology microbiome bifidobacteria Butyrate Gut flora medicine.disease_cause Microbiology digestive system 03 medical and health sciences chemistry.chemical_compound Anaerostipes caccae Lachnospiraceae medicine Monosaccharide Microbiome Food science MolEco Lactose VLAG chemistry.chemical_classification biology pH Carbohydrate biology.organism_classification butyrate 030104 developmental biology chemistry |
| Zdroj: | Beneficial Microbes, 12(1), 69-83 Beneficial Microbes 12 (2021) 1 Beneficial microbes, 12(1), 69. Wageningen Academic Publishers |
| ISSN: | 1876-2883 |
| Popis: | The establishment of the gut microbiota immediately after birth is a dynamic process that may impact lifelong health. At this important developmental stage in early life, human milk oligosaccharides (HMOs) serve as specific substrates to shape the gut microbiota of the nursling. The well-orchestrated transition is important as an aberrant microbial composition and bacterial-derived metabolites are associated with colicky symptoms and atopic diseases in infants. Here, we study the trophic interactions between an HMO-degrader, Bifidobacterium infantis and the butyrogenic Anaerostipes caccae using carbohydrate substrates that are relevant in the early life period including lactose and total human milk carbohydrates. Mono- and co-cultures of these bacterial species were grown at pH 6.5 in anaerobic bioreactors supplemented with lactose or total human milk carbohydrates. A. caccae was not able to grow on these substrates except when grown in co-culture with B. infantis, leading to growth and concomitant butyrate production. Two levels of cross-feeding were observed, in which A. caccae utilised the liberated monosaccharides as well as lactate and acetate produced by B. infantis. This microbial cross-feeding points towards the key ecological role of bifidobacteria in providing substrates for other important species that will colonise the infant gut. The progressive shift of the gut microbiota composition that contributes to the gradual production of butyrate could be important for host-microbial crosstalk and gut maturation. |
| Databáze: | OpenAIRE |
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