A new perfusion mode of culture for WJ‐MSCs expansion in a stirred and online monitored bioreactor
| Autor: | Bruno Ebel, Emmanuel Guedon, Fanny Gallo, Eric Olmos, Caroline Sion, Natalia de Isla, Dima Ghannoum, Isabelle Chevalot |
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| Přispěvatelé: | Laboratoire Réactions et Génie des Procédés (LRGP), Centre National de la Recherche Scientifique (CNRS)-Université de Lorraine (UL), Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Cell Culture Techniques Bioengineering 01 natural sciences Applied Microbiology and Biotechnology 03 medical and health sciences Bioreactors 010608 biotechnology Wharton's jelly Bioreactor Humans [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering ComputingMilieux_MISCELLANEOUS 030304 developmental biology Cell Proliferation 0303 health sciences Continuous flow Cell growth Chemistry Mesenchymal stem cell Microcarrier Cell Differentiation Mesenchymal Stem Cells On cells Perfusion Biotechnology Biomedical engineering |
| Zdroj: | Biotechnology and Bioengineering Biotechnology and Bioengineering, Wiley, 2021, 118 (11), pp.4453-4464. ⟨10.1002/bit.27914⟩ |
| ISSN: | 0006-3592 1097-0290 |
| DOI: | 10.1002/bit.27914⟩ |
| Popis: | Since a clinical dose requires a minimum of 106 cells per kilogram of patients, it is therefore crucial to develop a scalable method of production of Wharton Jelly Mesenchymal stem cells (WJ-MSCs) with maintained inner characteristics. Scalable expansion of WJ-MSCs on microcarriers usually found in cell culture, involved specific cell detachment using trypsin, and could have harmful effects on cells. In this work, performance of batch, fed-batch and perfused-continuous mode of culture were compared. The batch and fed-batch modes resulted in expansion factors of 5 and 43, respectively. The perfused-continuous mode strategy consisted in the implementation of a settling tube inside the bioreactor. The diameter of the tube was calculated to maintain microcarriers colonized by cells in the bioreactor whereas empty microcarriers (responsible of potentially damaging collisions) were removed, using a continuous flow rate based on MSCs physiological requirements. Thanks to this strategy, a maximal number of 800 million of cells was obtained in a 1.5 L bioreactor in 10 days. Lastly, on-line dielectric spectroscopy was implemented in the bioreactor and indicated that cell growth could be monitored during the culture. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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