Interferon-gamma increases monocyte PD-L1 but does not diminish T-cell activation
| Autor: | Susan Galandiuk, Hiram C. Polk, Campbell Bishop, Norman J. Galbraith, Samuel Walker, Sarah A. Gardner |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Adult Lipopolysaccharides Male Lipopolysaccharide medicine.medical_treatment T cell Immunology Programmed Cell Death 1 Receptor Gene Expression Immunopotentiator Biology CD8-Positive T-Lymphocytes Lymphocyte Activation B7-H1 Antigen Monocytes Sepsis 03 medical and health sciences chemistry.chemical_compound Interferon-gamma 0302 clinical medicine medicine Humans Interferon gamma Tumor Necrosis Factor-alpha Monocyte HLA-DR Antigens Middle Aged medicine.disease Acquired immune system Flow Cytometry 030104 developmental biology medicine.anatomical_structure Cytokine chemistry Cytokines Female 030215 immunology medicine.drug |
| Zdroj: | Cellular immunology. 357 |
| ISSN: | 1090-2163 |
| Popis: | Immune dysfunction can occur during sepsis or following major trauma. Decreased monocyte HLA-DR expression and cytokine responses are associated with mortality. Recent studies have shown that adaptive immune system defects can also occur in such patients, characterised by increased PD-L1 expression and associated T-cell anergy. The aim of this study was to determine the effects of an immune adjuvant, interferon-gamma, on monocyte PD-L1 expression and T-cell activation in an ex-vivo human whole blood model of infection. We found that with interferon-gamma treatment, monocytes had increased HLA-DR expression and augmented TNF-α production in response to LPS stimulation, with a decrease in IL-10 levels. Both LPS and interferon-gamma increased the level of monocyte PD-L1 expression, and that a combination of both agents synergistically stimulated a further increase in PD-L1 levels as measured by flow cytometry. However, despite elevated PD-L1 expression, both CD4 and CD8 T-cell activation was not diminished by the addition of interferon-gamma treatment. These findings suggest that PD-L1 may not be a reliable marker for T-cell anergy, and that interferon-gamma remains an adjuvant of interest that can improve the monocyte inflammatory response while preserving T-cell activation. |
| Databáze: | OpenAIRE |
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