Composition and electrophoretic mobility of plasma lipoproteins of dromedary camels (Camelus dromedarius)
| Autor: | Farzad Asadi, Mahzyar Samadaei, Peyman Asadian, Malihe Pourkabir, Ali Shahriari |
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| Rok vydání: | 2008 |
| Předmět: |
Plasma lipoprotein
Very low-density lipoprotein Camelus Chromatography General Veterinary Cholesterol General Medicine Lipoproteins VLDL Lipoproteins LDL Electrophoresis chemistry.chemical_compound chemistry Centrifugation Density Gradient Animals Electrophoresis Polyacrylamide Gel lipids (amino acids peptides and proteins) Centrifugation Composition (visual arts) Lipoproteins HDL Polyacrylamide gel electrophoresis Lipoprotein |
| Zdroj: | American Journal of Veterinary Research. 69:880-885 |
| ISSN: | 0002-9645 |
| DOI: | 10.2460/ajvr.69.7.880 |
| Popis: | Objective—To determine the lipid composition and electrophoretic pattern of plasma lipoproteins in samples obtained from healthy 1-humped camels (Camelus dromedarius). Animals—34 healthy camels raised under similar farming and dietary conditions. Procedures—Plasma samples were subjected to density-gradient ultracentrifugation for separation of plasma lipoproteins, including very-low-density lipoproteins (VLDL), low-density lipoproteins (LDL), and high-density lipoproteins (HDL). Purity of the separation was assessed by use of polyacrylamide gel disk electrophoresis. Concentrations of triglycerides, cholesterol, and phospholipids were measured in each lipoprotein fraction, and lipoprotein electrophoretic patterns were determined in plasma samples. Results—Phospholipid was the major constituent of VLDL (mean ± SD concentration, 10.62 ± 1.2 mg/dL), LDL (24.66 ± 3.12 mg/dL), and HDL (38.08 ± 0.76 mg/dL). Low-density lipoprotein, VLDL, and HDL were important plasma lipoprotein carriers for cholesterol (67.94 ± 9.51%), triglyceride (55.83 ± 7.81%), and phospholipid (51.91 ± 1.55%), respectively. On the basis of electrophoresis results, relative percentages of α- and β-lipoproteins were 31.72 ± 4.88% and 68.3 ± 4.68%, respectively. Conclusions and Clinical Relevance—The lipoprotein profile in 1-humped camels differed substantially from that of other ruminants. Results may be useful in the evaluation of metabolic disorders in camels. |
| Databáze: | OpenAIRE |
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