ATRA Entrapped in DSPC Liposome Enhances Anti-metastasis Effect on Lung and Liver During B16F10 Cell Line Metastasis in C57BL6 Mice
| Autor: | Reshma Mahima Reji, Berlin Grace V.M, Siddikuzzaman |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Cancer Research Pathology medicine.medical_specialty Lung Neoplasms Melanoma Experimental Retinoic acid Tretinoin 030226 pharmacology & pharmacy Metastasis Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cell Line Tumor medicine Animals Potency Lung cancer neoplasms Pharmacology Liposome Lung business.industry organic chemicals Cancer medicine.disease biological factors Mice Inbred C57BL Leukemia 030104 developmental biology medicine.anatomical_structure chemistry Liposomes Cancer research Molecular Medicine business |
| Zdroj: | Anti-Cancer Agents in Medicinal Chemistry. 17 |
| ISSN: | 1871-5206 |
| DOI: | 10.2174/1871520616666160927103458 |
| Popis: | Background: The high mortality rate of lung cancer is highly associated with faster metastasis spread. All Trans Retinoic Acid (ATRA), being the first choice drug for leukemia therapy is now under intense study for its therapeutic efficiency in other solid cancers. Objectives: This study was aimed to investigate the anti-metastasis activity of free ATRA and liposome entrapped ATRA (5:4:1) in the experimental C57BL/6 mice model developed by the injection of B16F10 cell line into the tail vein. Method: The ATRA drug was given via i.p for 21 days. The visual lung and liver metastatic tumor nodules were noted. Various biochemical markers of cancer metastasis in the serum as well as tissues were also analyzed after sacrifice. Results: Tumor nodules have significantly decreased in ATRA treatment groups (32.83 ± 1.83 for free ATRA, 23 ± 2.36 for DSPC Lipo-ATRA) when compared with metastasis control (63.16 ± 2.9) in the lungs. Among the treatment groups, the DSPC lipo-ATRA treated group showed a significant tumor growth inhibition (63.6%) than that of in the free ATRA treated groups (48%). Similar anti-metastatic effect was observed in liver also. Furthermore lipo-ATRA has shown a significant change in the levels of biochemical cancer markers analyzed in this study. Conclusion: Our results concluded that the liposome encapsulated ATRA has an enhanced anti-metastasis potency than the free ATRA during B16F10 metastatic cell line implantation. |
| Databáze: | OpenAIRE |
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