Identification of a Nuclear Exosome Decay Pathway for Processed Transcripts
| Autor: | Yun Chen, Coline Gentil, Kristoffer Vitting-Seerup, Albin Sandelin, Nicola Meola, Evdoxia Karadoulama, Søren Lykke-Andersen, Michal Domanski, Torben Heick Jensen, Dennis Pultz, Jens S. Andersen |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Polyadenylation Exosome complex RNA Stability Biology Mutually exclusive events Poly(A)-Binding Protein I Exosome 03 medical and health sciences 0302 clinical medicine Humans RNA Messenger RNA Small Interfering Molecular Biology Cell Nucleus Messenger RNA Binding Sites Exosome Multienzyme Ribonuclease Complex Nuclear Proteins RNA-Binding Proteins RNA Cell Biology Molecular biology Cell biology HEK293 Cells 030104 developmental biology TRAMP complex Carrier Proteins Poly A RNA Helicases 030217 neurology & neurosurgery HeLa Cells Protein Binding Transcription Factors |
| Zdroj: | Meola, N, Domanski, M, Karadoulama, E, Chen, Y, Gentil, C, Pultz, D, Vitting-Seerup, K, Lykke-Andersen, S, Andersen, J S, Sandelin, A & Jensen, T H 2016, ' Identification of a Nuclear Exosome Decay Pathway for Processed Transcripts ', Molecular Cell, vol. 64, no. 3, pp. 520-533 . https://doi.org/10.1016/j.molcel.2016.09.025 Meola, N, Domanski, M, Karadoulama, E, Chen, Y, Gentil, C, Pultz, D, Vitting-Seerup, K, Lykke-Andersen, S, Andersen, J S, Sandelin, A G & Jensen, T H 2016, ' Identification of a Nuclear Exosome Decay Pathway for Processed Transcripts ', Molecular Cell, vol. 64, no. 3, pp. 520-533 . https://doi.org/10.1016/j.molcel.2016.09.025 |
| ISSN: | 1097-2765 |
| DOI: | 10.1016/j.molcel.2016.09.025 |
| Popis: | The RNA exosome is fundamental for the degradation of RNA in eukaryotic nuclei. Substrate targeting is facilitated by its co-factor Mtr4p/hMTR4, which links to RNA-binding protein adaptors. One example is the trimeric human nuclear exosome targeting (NEXT) complex, which is composed of hMTR4, the Zn-finger protein ZCCHC8, and the RNA-binding factor RBM7. NEXT primarily targets early and unprocessed transcripts, which demands a rationale for how the nuclear exosome recognizes processed RNAs. Here, we describe the poly(A) tail exosome targeting (PAXT) connection, which comprises the ZFC3H1 Zn-knuckle protein as a central link between hMTR4 and the nuclear poly(A)-binding protein PABPN1. Individual depletion of ZFC3H1 and PABPN1 results in the accumulation of common transcripts that are generally both longer and more extensively polyadenylated than NEXT substrates. Importantly, ZFC3H1/PABPN1 and ZCCHC8/RBM7 contact hMTR4 in a mutually exclusive manner, revealing that the exosome targets nuclear transcripts of different maturation status by substituting its hMTR4-associating adaptors. © 2016 Elsevier Inc. |
| Databáze: | OpenAIRE |
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