Use of LAMP for Assessing Botrytis cinerea Colonization of Bunch Trash and Latent Infection of Berries in Grapevines
| Autor: | Vittorio Rossi, Melissa Si Ammour, Giorgia Fedele, Eleonora Castaldo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Loop-mediated isothermal amplification Plant Science crude extract 01 natural sciences 03 medical and health sciences Colonization on-site testing Ecology Evolution Behavior and Systematics Botrytis cinerea Ecology biology Inoculation fungi Botany food and beverages Ripening biology.organism_classification real-time monitoring eye diseases Horticulture 030104 developmental biology QK1-989 Botrytis bunch rot Settore AGR/12 - PATOLOGIA VEGETALE loop-mediated isothermal amplification 010606 plant biology & botany |
| Zdroj: | Plants, Vol 9, Iss 1538, p 1538 (2020) Plants Volume 9 Issue 11 |
| ISSN: | 2223-7747 |
| Popis: | A real-time loop-mediated isothermal amplification (LAMP) assay was evaluated for the detection of Botrytis cinerea in grapevine bunch trash, immature berries, and ripening berries. A simple method for the preparation of crude extracts of grape tissue was also developed for on-site LAMP analysis. When tested with 14 other fungal species frequently found in grapevines, the LAMP assay was specific and sensitive to a B. cinerea DNA quantity of 0.1 ng/µ L. The sensitivity was further tested using bunch trash samples with B. cinerea colonization levels between 6 and 100% and with bulk-berry samples composed of 4 pathogen-free berries or 4 berries among which 25 to 100% had been inoculated with B. cinerea. The LAMP assay detected the lowest B. cinerea colonization level tested in bunch trash and in immature and mature berries in less than 20 min. In single-berry experiments, LAMP amplified B. cinerea DNA from all artificially inoculated individual immature and mature berries. No amplification occurred in B. cinerea-free material. The real-time LAMP assay has the potential to be used as a rapid on-site diagnostic tool for assessing B. cinerea colonization in bunch trash and B. cinerea latent infections in berries, which represent critical stages for decision-making about disease management. |
| Databáze: | OpenAIRE |
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