Biofilm Induced Profiles of Immune Response Gene Expression by Oral Epithelial Cells

Autor: Rebecca Peyyala, Octavio A. Gonzalez, Jeffrey L. Ebersole
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Microbiology (medical)
Immunology
Interleukin-1beta
Gingiva
Down-Regulation
Gene Expression
CD59 Antigens
Adaptive Immunity
Microbiology
Article
03 medical and health sciences
0302 clinical medicine
NF-KappaB Inhibitor alpha
Transforming Growth Factor beta
Interleukin-1alpha
Humans
Interleukin 8
RNA
Messenger
General Dentistry
Porphyromonas gingivalis
Extracellular Matrix Proteins
Mouth
Immune response gene
Chemokine CCL20
biology
Fusobacterium nucleatum
Interleukins
Microbiota
CD44
Interleukin-8
Streptococcus gordonii
Biofilm
Epithelial Cells
030206 dentistry
biochemical phenomena
metabolism
and nutrition
biology.organism_classification
Acquired immune system
Immunity
Innate
Up-Regulation
030104 developmental biology
Hyaluronan Receptors
Biofilms
biology.protein
Transcriptome
Popis: OBJECTIVE: This study examined the oral epithelial immunotranscriptome response patterns modulated by oral bacterial planktonic or biofilm challenge. METHODS: We assessed gene expression patterns when epithelial cells were challenged with a multispecies biofilm composed of S. gordonii, F. nucleatum, and P. gingivalis representing a type of periodontopathic biofilm compared to challenge with the same species of planktonic bacteria. RESULTS: Of the 579 human immunology genes, a substantial signal of the epithelial cells was observed to 181 genes. Biofilm challenged stimulated significant elevations compared to planktonic bacteria for IL32, IL8, CD44, B2M, TGFBI, NFKBIA, IL1B, CD59, IL1A, CCL20 representing the top 10 signals comprising 55% of the overall signal for the epithelial cell responses. Levels of PLAU, CD9, IFITM1, PLAUR, CD24, TNFSF10, and IL1RN were all elevated by each of the planktonic bacterial challenge versus the biofilm responses. While the biofilms upregulated 123/579 genes (>2-fold), fewer genes were increased by the planktonic species [36 (S. gordonii), 30 (F. nucleatum), 44 (P. gingivalis)]. CONCLUSIONS: A wide array of immune genes were regulated by oral bacterial challenge of epithelial cells that would be linked to the local activity of innate and adaptive immune response components in the gingival tissues. Incorporating bacterial species into a structured biofilm dramatically altered the number and level of genes expressed. Additionally a specific set of genes were significantly decreased with the multispecies biofilms suggesting that some epithelial cell biologic pathways are down-regulated when in contact with this type of pathogenic biofilm.
Databáze: OpenAIRE
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