Surface Probe Linker with Tandem Anti-Fouling Properties for Application in Biosensor Technology
| Autor: | Michael Thompson, Tibor Hianik, Brian De La Franier, Sandro Spagnolo |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Materials science
lcsh:Biotechnology Clinical Biochemistry Nanotechnology 02 engineering and technology Biosensing Techniques 010402 general chemistry 01 natural sciences Article anti-fouling monolayer Blood serum lcsh:TP248.13-248.65 Monolayer Humans milk Fouling Tandem General Medicine Propanoyl chloride 021001 nanoscience & nanotechnology Piezoelectricity 0104 chemical sciences electromagnetic piezoelectric acoustic sensor blood serum Molecular Probes 0210 nano-technology Linker Biosensor |
| Zdroj: | Biosensors Biosensors, Vol 10, Iss 3, p 20 (2020) Volume 10 Issue 3 |
| ISSN: | 2079-6374 |
| Popis: | This paper describes the anti-fouling capability of the novel monolayer-forming surface linker 3-(3-(trichlorosilylpropyloxy) propanoyl chloride (MEG-Cl). This compound was successfully attached to quartz crystal surfaces which are employed in an electromagnetic piezoelectric acoustic sensor (EMPAS) configuration. The MEG-Cl coated surface was both employed with Ni-NTA for the binding of recombinant proteins and for the tandem property of the avoidance of fouling from serum and milk. The MEG-Cl coated surfaces were found to provide a large degree of anti-fouling on the EMPAS device, and were comparable to previously studied MEG-OH surfaces. Importantly, the monolayer continued to provide anti-fouling capability to the biosensor following extension with Ni-NTA in place. Accordingly, this surface linker provides an attractive system for use in biosensor technology in terms of both its anti-fouling and linking properties. |
| Databáze: | OpenAIRE |
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