Recruitment and Collection of Dermal Interstitial Fluid Using a Microneedle Patch
| Autor: | Chandana Kolluru, Mark R. Prausnitz, Jeremy Chae, Mikayla Williams |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Medical diagnostic
Chemistry Swine Biomedical Engineering Pharmaceutical Science Extracellular Fluid 02 engineering and technology Dermis 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Article 0104 chemical sciences Rats Biomaterials Skin hydration Interstitial fluid Needles Skin surface Animals 0210 nano-technology Biomedical engineering |
| Popis: | Interstitial fluid (ISF) that surrounds cells in tissues of the body is a novel source of biomarkers that complements conventional sources like blood, urine and saliva. To overcome difficulties in harvesting ISF, we developed a minimally invasive, rapid, simple-to-use and cost-effective method to collect ISF from the skin involving a microneedle (MN) patch. By pressing 650 μm long MNs at an angle just below the skin surface, blood-free ISF flowed through micropores to the skin surface and was absorbed into a thin strip of paper on the MN patch backing for subsequent analysis. An optimized method in rat skin in vivo was well tolerated and able to collect >2 μl of ISF within 1 min. Brief skin pre-treatment with MNs followed by a 5 min delay dramatically increased subsequent ISF collection by a mechanism believed to involve increased skin hydration. ISF collection using a MN patch has the potential to simplify access to biomarkers in ISF for research and future medical diagnostic and monitoring applications. |
| Databáze: | OpenAIRE |
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