Secreted antigens of the amastigote and promastigote forms of Leishmania infantum inducing a humoral response in humans and dogs
| Autor: | P. Cibrelus, B. Carcy, E. Précigout, A. Gorenflot, Denis Sereno, J.L. Lemesre |
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| Rok vydání: | 1999 |
| Předmět: |
Veterinary (miscellaneous)
Antibodies Protozoan Antigens Protozoan Microbiology Immune system Dogs Antigen Animals Humans Dog Diseases Leishmania infantum Amastigote biology Kinetoplastida biology.organism_classification Precipitin Tests In vitro Infectious Diseases Insect Science Humoral immunity Immunology Antibody Formation Protozoa Leishmaniasis Visceral Animal Science and Zoology Parasitology |
| Zdroj: | Parasite (Paris, France). 6(2) |
| ISSN: | 1252-607X |
| Popis: | To study the antigens secteted by promastigote and amastigote forms of Leishmania infantum which are able to induce a humoral response in human patients and dogs, we have carried out immunoprecipitation assays with different supernatants of in vitro cultured parasites, metabolically labelled with [35 S] methionine, using serum samples from human patients and dogs. In addition, some metabolic labelling experiments were performed daily during the in vitro culture parasite's life cycle to follow the time course excretion-secretion of parasitic antigens. The results demonstrated that the two different hosts developed an antibody response against secreted antigens of both stages of Leishmania infantum . Nevertheless, the humoral response directed against the excreted-secreted antigens of the promastigote forms was qualitatively and quantitatively different when we compare the human and the dog immune responses. On the other hand, when the excreted-secteted antigens of the amastigote forms are immunoprecipitated with eithet human or canine immune serum, the humoral response is similar. In addition, the time course study showed that excretion-secretion of antigens was qualitatively and quantitatively modulated during the parasitic in vitro life cycle. |
| Databáze: | OpenAIRE |
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