Pathological features of rat lung following inhalation and intratracheal instillation of C60fullerene

Autor:	Shigehisa Endoh, Isamu Tanaka, Masahiro Murakami, Naohide Shinohara, Wei-Ning Wang, Manabu Shimada, Akira Ogami, Junko Nakanishi, Kunio Uchida, Takako Oyabu, Masami Hirohashi, Toshihiko Myojo, Makoto Yamamoto, Yasuo Morimoto, Kazuhiro Yamamoto, Kenichiro Nishi, Motoi Todoroki, Katsuhide Fujita, Chikara Kadoya
Rok vydání:	2011
Předmět:	Male Intratracheal instillation Pulmonary toxicity Health Toxicology and Mutagenesis Observation period Metal Nanoparticles Toxicology medicine Animals Particle Size Rats Wistar Lung Pathological Inflammation Inhalation Exposure No-Observed-Adverse-Effect Level Inhalation business.industry Lung Injury Rats medicine.anatomical_structure Point counting Anesthesia Fullerenes Early phase business
Zdroj:	Inhalation Toxicology. 23:407-416
ISSN:	1091-7691 0895-8378
DOI:	10.3109/08958378.2011.580386
Popis:	We evaluated the pulmonary pathological features of rats that received a single intratracheal instillation and a 4-week inhalation of a fullerene. We used fullerene C(60) (nanom purple; Frontier Carbon Co. Ltd, Japan) in this study. Male Wistar rats received intratracheal dose of 0.1, 0.2, or 1 mg of C(60), and were sacrificed at 3 days, 1 week, 1 month, 3 months, 6 months, and 12 months. In the inhalation study, Wistar rats received C(60) or nickel oxide by whole-body inhalation for 6 h/day, 5 days/week, 4 weeks, and were sacrificed at 3 days, 1 month, and 3 months after the end of exposure. During the observation period, no tumors or granulomas were observed in either study. Histopathological evaluation by the point counting method (PCM) showed that a high dose of C(60) (1 mg) instillation led to a significant increase of areas of inflammation in the early phase (until 1 week). In the inhalation study of the C(60)-exposed group, PCM evaluation showed significant changes in the C(60)-exposed group only at 3 days after exposure; after 1 month, no significant changes were observed. The present study demonstrated that the pulmonary inflammation pattern after exposure to well-characterized C(60) via both intratracheal and inhalation instillation was slight and transient. These results support our previous studies that showed C(60) has no significant adverse effects in intratracheal and inhalation instillation studies.
Databáze:	OpenAIRE
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