The incorporation of [14C]acetate into the constituent fatty acids of monogalactosyldiglyceride by isolated spinach chloroplasts
| Autor: | J.C. Hawke, J.W.A. Mckee |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
Chloroplasts
Chromatography Galactolipids Fatty Acids Biophysics Acetates Plants Biochemistry Glycerides Polyethylene Glycols Diglycerides Uridine Diphosphate Galactose 14c acetate chemistry.chemical_compound Biosynthesis chemistry Glycerophosphates Glycerol Glycolipids Spinach chloroplast Molecular Biology |
| Zdroj: | Archives of Biochemistry and Biophysics. 197:322-332 |
| ISSN: | 0003-9861 |
| DOI: | 10.1016/0003-9861(79)90252-2 |
| Popis: | The incorporation into diglycerides of the acyl products synthesized from acetate by spinach chloroplasts was greatly stimulated by the addition of glycerol 3-phosphate. When UDP-galactose was added also, monogalactosyldiglycerides became the major products. Palmitate biosynthesis was stimulated about twofold by these additions, while oleate biosynthesis decreased slightly, so that oleate:palmitate ratios were in the range 0.6 to 0.8 rather than about 1.6 when glycerol 3-phosphate and UDP-galactose were not added. On the other hand, Triton X-100 greatly stimulated both oleate and palmitate biosynthesis to give oleate:palmitate ratios of about 2.0. The proportions of oleate and palmitate in the newly synthesized diglycerides, or in monogalactosyldiglycerides when exogenous UDP-galactose was added, did not always reflect the proportions of these two fatty acids synthesized from acetate. When oleate:palmitate ratios were ⩾1, equal amounts were incorporated into diglycerides or into monogalactosyldiglycerides. When oleate:palmitate ratios were |
| Databáze: | OpenAIRE |
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