A Review on the Effects of Supercritical Carbon Dioxide on Enzyme Activity

Autor: Marie Zarevúcka, Zdenĕk Wimmer
Rok vydání: 2010
Předmět:
Zdroj: International Journal of Molecular Sciences, Vol 11, Iss 1, Pp 233-253 (2010)
International Journal of Molecular Sciences
ISSN: 1422-0067
DOI: 10.3390/ijms11010233
Popis: Different types of enzymes such as lipases, several phosphatases, dehydrogenases, oxidases, amylases and others are well suited for the reactions in SC-CO(2). The stability and the activity of enzymes exposed to carbon dioxide under high pressure depend on enzyme species, water content in the solution and on the pressure and temperature of the reaction system. The three-dimensional structure of enzymes may be significantly altered under extreme conditions, causing their denaturation and consequent loss of activity. If the conditions are less adverse, the protein structure may be largely retained. Minor structural changes may induce an alternative active protein state with altered enzyme activity, specificity and stability.
Databáze: OpenAIRE