Karyotyping human and mouse cells using probes from single-sorted chromosomes and open source software
Autor: | Shamilene Sivagnanam, William D. Bradford, Tamara A. Potapova, Rong Li, Yuping Wu, Christopher Seidel, Brian D. Slaughter, Jay R. Unruh, Andrew C. Box |
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Rok vydání: | 2015 |
Předmět: |
medicine.medical_specialty
Microscope Laser scanning Confocal Multispectral image Aneuploidy Biology General Biochemistry Genetics and Molecular Biology Cell Line law.invention Mice law medicine Animals Chromosomes Human Humans Genetics business.industry Cytogenetics Karyotype Pattern recognition medicine.disease Chromosomes Mammalian Spectral imaging Karyotyping Artificial intelligence business Software Biotechnology |
Zdroj: | BioTechniques. 59:335-346 |
ISSN: | 1940-9818 0736-6205 |
Popis: | Multispectral karyotyping analyzes all chromosomes in a single cell by labeling them with chromosome-specific probes conjugated to unique combinations of fluorophores. Currently available multispectral karyotyping systems require the purchase of specialized equipment and reagents. However, conventional laser scanning confocal microscopes that are capable of separating multiple overlapping emission spectra through spectral imaging and linear unmixing can be utilized for classifying chromosomes painted with multicolor probes. Here, we generated multicolor chromosome paints from single-sorted human and mouse chromosomes and developed the Karyotype Identification via Spectral Separation (KISS) analysis package, a set of freely available open source ImageJ tools for spectral unmixing and karyotyping. Chromosome spreads painted with our multispectral probe sets can be imaged on widely available spectral laser scanning confocal microscopes and analyzed using our ImageJ tools. Together, our probes and software enable academic labs with access to a laser-scanning spectral microscope to perform multicolor karyotyping in a cost-effective manner. |
Databáze: | OpenAIRE |
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