Primed histone demethylation regulates shoot regenerative competency
| Autor: | Takuya Sakamoto, Mitsuhiro Aida, Haruka Temman, Kengo Morohashi, Tetsuji Kakutani, Hiroya Ishihara, Sachihiro Matsunaga, Kaoru Sugimoto, Motoaki Seki, Taku Sasaki, Elliot M. Meyerowitz, Paul T. Tarr, Yayoi Inui, Soichi Inagaki, Takamasa Suzuki, Satoshi Kadokura |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cell type Science Arabidopsis General Physics and Astronomy Priming (immunology) 02 engineering and technology General Biochemistry Genetics and Molecular Biology Article Epigenesis Genetic Histones 03 medical and health sciences Histone demethylation Gene Expression Regulation Plant Plant Cells Gene silencing Regeneration Induced pluripotent stem cell lcsh:Science Regulation of gene expression Histone Demethylases Multidisciplinary biology Arabidopsis Proteins fungi food and beverages General Chemistry 021001 nanoscience & nanotechnology Plants Genetically Modified Cell biology Demethylation Histone Code 030104 developmental biology Histone biology.protein Demethylase lcsh:Q 0210 nano-technology Protein Processing Post-Translational Plant Shoots |
| Zdroj: | Nature Communications, Vol 10, Iss 1, Pp 1-15 (2019) Nature Communications |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-019-09386-5 |
| Popis: | Acquisition of pluripotency by somatic cells is a striking process that enables multicellular organisms to regenerate organs. This process includes silencing of genes to erase original tissue memory and priming of additional cell type specification genes, which are then poised for activation by external signal inputs. Here, through analysis of genome-wide histone modifications and gene expression profiles, we show that a gene priming mechanism involving LYSINE-SPECIFIC DEMETHYLASE 1-LIKE 3 (LDL3) specifically eliminates H3K4me2 during formation of the intermediate pluripotent cell mass known as callus derived from Arabidopsis root cells. While LDL3-mediated H3K4me2 removal does not immediately affect gene expression, it does facilitate the later activation of genes that act to form shoot progenitors when external cues lead to shoot induction. These results give insights into the role of H3K4 methylation in plants, and into the primed state that provides plant cells with high regenerative competency. Plant regeneration can occur via formation of a mass of pluripotent cells known as callus. Here, Ishihara et al. show that acquisition of regenerative capacity of callus-forming cells requires a lysine-specific demethylase that removes H3K4me2 to prime gene expression in response to regenerative cues. |
| Databáze: | OpenAIRE |
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