Expression and Functional Characterization of aDrosophila Neuropeptide Precursor with Homology to Mammalian Preprotachykinin A
| Autor: | Geoffrey M. Coast, Åsa M.E. Winther, Dick R. Nässel, Alan D. Shirras, R. Elwyn Isaac, Ronald J. Nachman, David Coates, Richard J. Siviter, Christine A.M. Taylor |
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| Rok vydání: | 2000 |
| Předmět: |
DNA
Complementary animal structures Tachykinin peptides Molecular Sequence Data Enteroendocrine cell complex mixtures digestive system Biochemistry Tachykinins Complementary DNA Animals Drosophila Proteins Protein Precursors Molecular Biology Mammals Base Sequence Sequence Homology Amino Acid biology cDNA library musculoskeletal neural and ocular physiology fungi Cell Biology biology.organism_classification Molecular biology Cell biology Open reading frame Drosophila Drosophila melanogaster Tachykinin receptor Drosophila Protein |
| Zdroj: | Journal of Biological Chemistry. 275:23273-23280 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m002875200 |
| Popis: | Peptides structurally related to mammalian tachykinins have recently been isolated from the brain and intestine of several insect species, where they are believed to function as both neuromodulators and hormones. Further evidence for the signaling role of insect tachykinin-related peptides was provided by the cloning and characterization of cDNAs for two tachykinin receptors from Drosophila melanogaster. However, no endogenous ligand has been isolated for the Drosophila tachykinin receptors to date. Analysis of the Drosophila genome allowed us to identify a putative tachykinin-related peptide prohormone (prepro-DTK) gene. A 1.5-kilobase pair cDNA amplified from a Drosophila head cDNA library contained an 870-base pair open reading frame, which encodes five novel Drosophila tachykinin-related peptides (called DTK peptides) with conserved C-terminal FXGXR-amide motifs common to other insect tachykinin-related peptides. The tachykinin-related peptide prohormone gene (Dtk) is both expressed and post-translationally processed in larval and adult midgut endocrine cells and in the central nervous system, with midgut expression starting at stage 17 of embryogenesis. The predicted Drosophila tachykinin peptides have potent stimulatory effects on the contractions of insect gut. These data provide additional evidence for the conservation of both the structure and function of the tachykinin peptides in the brain and gut during the course of evolution. |
| Databáze: | OpenAIRE |
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