Profiling and quantitative analysis of underivatized fatty acids in Chlorella vulgaris microalgae by liquid chromatography‐high resolution mass spectrometry
| Autor: | Chiara Cavaliere, Susy Piovesana, Aldo Laganà, Sara Elsa Aita, Andrea Cerrato, Carmela Maria Montone, Martina Catani, Anna Laura Capriotti |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Analyte Chlorella vulgaris Palmitic Acid Filtration and Separation 010501 environmental sciences 01 natural sciences fatty acids Mass Spectrometry Analytical Chemistry NO Cell wall Fatty Acids Monounsaturated Linoleic Acid Hydrolysis 010608 biotechnology biofuel compound discoverer lipidomics untargeted analysis Lipidomics Microalgae Hexanes Biomass 0105 earth and related environmental sciences Liquid Chromatography Chromatography Chemistry Extraction (chemistry) Temperature Reproducibility of Results alpha-Linolenic Acid Lipids Biofuels Calibration Acid hydrolysis biofuel compound discoverer fatty acids lipidomics untargeted analysis Quantitative analysis (chemistry) Stearic Acids Research Article Chromatography Liquid Oleic Acid |
| Zdroj: | Journal of Separation Science |
| ISSN: | 1615-9314 1615-9306 |
| Popis: | Chlorella vulgaris is a popular microalga used for biofuel production; nevertheless, it possesses a strong cell wall that hinders the extraction of molecules, especially lipids within the cell wall. For tackling this issue, we developed an efficient and cost‐effective method for optimal lipid extraction. Microlaga cell disruption by acid hydrolysis was investigated comparing different temperatures and reaction times; after hydrolysis, lipids were extracted with n‐hexane. The best recoveries were obtained at 140°C for 90 min. The microalgae were then analyzed by an untargeted approach based on liquid chromatography with high‐resolution mass spectrometry, providing the tentative identification of 28 fatty acids. First, a relative quantification on the untargeted data was performed using peak area as a surrogate of analyte abundance. Then, a targeted quantitative method was validated for the tentatively identified fatty acids, in terms of recovery (78‐100%), intra‐ and interday relative standard deviations ( 0.98). The most abundant fatty acids were palmitic, palmitoleic, oleic, linoleic, linolenic, and stearic acids. |
| Databáze: | OpenAIRE |
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