The influence of local and systemic preconditioning on oxygenation, metabolism and survival in critically ischaemic skin flaps in pigs
| Autor: | Claudio Contaldo, Dominique Erni, A. Banic, Stephan M. Jakob, Jan A. Plock, Yves Harder |
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| Rok vydání: | 2007 |
| Předmět: |
Microdialysis
medicine.medical_specialty Lipid A/administration & dosage/analogs & derivatives/physiology Swine Ischemia Surgical Flaps Heat shock protein Surgical Flaps/blood supply Laser-Doppler Flowmetry medicine Animals HSP70 Heat-Shock Proteins Ischemic Preconditioning/methods Ischemic Preconditioning Analysis of Variance ddc:617 Heat-Shock Response/physiology business.industry Swine/anatomy & histology Metabolism Blood flow Oxygenation medicine.disease Ischemia/chemically induced Hsp70 Surgery Lipid A Systemic administration business Heat-Shock Response HSP70 Heat-Shock Proteins/physiology |
| Zdroj: | Journal of Plastic, Reconstructive & Aesthetic Surgery, Vol. 60, No 11 (2007) pp. 1182-92 |
| ISSN: | 1748-6815 |
| DOI: | 10.1016/j.bjps.2007.02.011 |
| Popis: | Stress proteins represent a group of highly conserved intracellular proteins that provide adaptation against cellular stress. The present study aims to elucidate the stress protein-mediated effects of local hyperthermia and systemic administration of monophosphoryl lipid A (MPL) on oxygenation, metabolism and survival in bilateral porcine random pattern buttock flaps. Preconditioning was achieved 24h prior to surgery by applying a heating blanket on the operative site (n = 5), by intravenous administration of MPL at a dosage of 35 microg/kg body weight (n = 5) or by combining the two (n = 5). The flaps were monitored with laser Doppler flowmetry, polarographic microprobes and microdialysis until 5h postoperatively. Semiquantitative immunohistochemistry was performed for heat shock protein 70 (HSP70), heat shock protein 32 (also termed haem oxygenase-1, HO-1), and inducible nitrc oxide synthase (iNOS). The administration of MPL increased the impaired microcirculatory blood flow in the proximal part of the flap and partial oxygen tension in the the distal part by approximately 100% each (both P |
| Databáze: | OpenAIRE |
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