In vitro osteodifferentiation of intact human amniotic membrane is not beneficial in the context of bone repair

Autor: Aurélie Nallet, Laurent Obert, Benoit de Billy, Florelle Gindraux, Mathilde Fenelon, C. Meyer, Virginie Moutarlier, R. Laurent, Fabienne Pouthier, Thomas Gualdi
Přispěvatelé: Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), Nanomédecine, imagerie, thérapeutique - UFC (EA 4662) (NIT / NANOMEDECINE), Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Univers, Transport, Interfaces, Nanostructures, Atmosphère et environnement, Molécules (UMR 6213) (UTINAM), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS), Bioingénierie tissulaire (BIOTIS), Université de Bordeaux (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM), NOVOTEC, Service de Chirurgie Orthopédique Traumatologique et Plastique [Besançon], Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon)-Université de Franche-Comté (UFC), Service de Chirurgie d’Orthopédie Pédiatrique [CHRU Besançon], Service de Chirurgie Maxillo-Faciale et Stomatologie
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Bone Regeneration
Biomedical Engineering
Cell Culture Techniques
Bone healing
Tissue Banks
[SDV.MHEP.CHI]Life Sciences [q-bio]/Human health and pathology/Surgery
Regenerative Medicine
Regenerative medicine
Osteocytes
Bone and Bones
Biomaterials
03 medical and health sciences
Tissue culture
0302 clinical medicine
Osteogenesis
medicine
media_common.cataloged_instance
Humans
[CHIM]Chemical Sciences
Amnion
European union
ComputingMilieux_MISCELLANEOUS
media_common
030222 orthopedics
Transplantation
Bone Transplantation
Osteoblasts
Tissue Engineering
Tissue Scaffolds
Chemistry
Mesenchymal stem cell
food and beverages
virus diseases
Cell Differentiation
Epithelial Cells
Mesenchymal Stem Cells
Cell Biology
In vitro
3. Good health
Cell biology
medicine.anatomical_structure
Orthopedics
Phenotype
Cell culture
Osteocyte
030221 ophthalmology & optometry
Zdroj: Cell and Tissue Banking
Cell and Tissue Banking, Springer Verlag, 2019, 20 (3), pp.435-446. ⟨10.1007/s10561-019-09778-3⟩
ISSN: 1389-9333
1573-6814
Popis: The human amniotic membrane (hAM) is an attractive biomaterial for regenerative medicine, as it contains amniotic mesenchymal stromal cells (hAMSC), epithelial cells (hAEC) and growth factors. We examined the potential use of hAM in orthopaedic and maxillofacial bone surgery, integrating the requirements of current regulations regarding advanced therapy medicinal products (ATMP) in the European Union. Previous studies have described the potential osteodifferentiation of intact hAM during whole-tissue culture in osteogenic conditions. The present study aims to determine whether in vitro osteodifferentiation of hAM is needed in the context bone repair, and the influence of this process on tissue structure, cell phenotype and cell function. Different conditions (fresh or cultured hAM; intact or hAM-derived cells) were tested. Phenotypic and functional analyses were performed with standard approaches (cell culture and staining, histological and immunolabelling) as well as original approaches (tissue staining, energy dispersive X-ray and X-ray diffraction). In our study, non-osteodifferentiated hAM (i.e., fresh or native hAM) exhibited innate pre-osteoblastic potential. Osteodifferentiation of fresh hAM induced a change in tissue structure, cell phenotype and function. Therefore, we hypothesize that pre-osteodifferentiation may not be necessary, especially if it induces unwanted changes. To our surprise, in these osteogenic conditions, hAEC had a mesenchymal phenotype with osteocyte function, and even native synthesis of hydroxyapatite, focusing osteogenic potential mainly in this epithelial layer. In conclusion, in vitro osteodifferentiation by tissue culture does not appear to be necessary for hAM to be used as an innovative ATMP for bone repair.
Databáze: OpenAIRE
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