| Popis: |
According to several investigation carried out about effective proteins in viper's venoms, two major families of protein, Dendrotoxins and Protease Inhibitors, has been recognized. It seems that they have been derived from the same ancestor gene which has been undergone several mutations. The chosen protein for this investigation, PPTI, has intermediate characteristics which can represent it as an appropriate choice for finding a relation between mentioned family groups.In this study, in order to achieve the three dimensional structure of PPTI, 2D-NMR spectroscopy was applied in which NOESY, TOCSY and HSQC spectra has been acquired. Conventional peak assignment was applied to identify amino acids and their contacts. |
