Additional file 3 of Long-term live imaging and multiscale analysis identify heterogeneity and core principles of epithelial organoid morphogenesis

Autor: Hof, Lotta, Moreth, Till, Koch, Michael, Liebisch, Tim, Kurtz, Marina, Tarnick, Julia, Lissek, Susanna M., Verstegen, Monique M. A., Laan, Luc J. W. Van Der, Meritxell Huch, Matthäus, Franziska, Stelzer, Ernst H. K., Pampaloni, Francesco
Rok vydání: 2021
DOI: 10.6084/m9.figshare.14111614.v1
Popis: Additional file 3: Fig. S2. Ultra-thin FEP-foil cuvette holders for live recordings with the Zeiss Lightsheet Z.1 microscope system. (a) Illustration of the general setup of the Zeiss Lightsheet Z.1 microscope. (b) Close-up of the microscope chamber with the downwards directed Z1-FEP-cuvette enclosing the sample. (c) Close-up of the sample holder. The shrinking tube that seals the FEP cuvette and connects it with the glass capillary is depicted in black. (d) CAD-derived drawings of positive moulds of the FEP cuvette and the glass capillary needed to produce the Z1-FEP-cuvette. (e) Printed mould with a glass capillary used to form the Z1-FEP-cuvette in the vacuum forming process. (f) Ready-to-use Z1-FEP-cuvette. (g) mPOs grown for 7 days in the Z1-FEP-cuvette.
Databáze: OpenAIRE