Cluster K Mycobacteriophages: Insights into the Evolutionary Origins of Mycobacteriophage TM4
| Autor: | Paras Jain, Kurt E. Williamson, Welkin H. Pope, Marilyn D. Jalal, Sarah C. R. Elgin, Graham F. Hatfull, Daniel A. Russell, Emilie G. Weisser, Margaret S. Saha, John T. Wertz, Forrest R. Guilfoile, Randall J. DeJong, Bo Zhang, Mariana Piuri, Molly J. McDonough, Steven G. Cresawn, Arrykka S. Jackson, Christopher D. Shaffer, Samuel E. Harvey, Lee E. Hughes, Deborah Jacobs-Sera, Kathleen Weston-Hafer, Robert C. Benjamin, Mark H. Forsyth, Coreen M. Manley, Stephanie E. Simon, Christina M. Ferreira, Corwin N. Rhyan, Peter M. Hynes, H. S. Rabinowitz, Elizabeth A. MacMurray, Roger W. Hendrix, Alexander D. Harris, Ariangela J. Davis, William R. Jacobs, Erika Fieldman Sims, Larissa J. Osterbann, Jordan L. Mosier, Isabel G. Tovar |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Bacterial Diseases
viruses lcsh:Medicine Virus Replication Genome Conserved sequence purl.org/becyt/ford/1 [https] Lysogen Viral classification Cluster Analysis Bacteriophages Genome Sequencing lcsh:Science Genome Evolution Conserved Sequence Genetics Multidisciplinary Mycobacteriophages biology Mycobacteriophage Mycobacterium smegmatis Temperature Chromosome Mapping Genomics Infectious Diseases Medical Microbiology Multigene Family Attachment Sites Microbiological Medicine CIENCIAS NATURALES Y EXACTAS Research Article Otras Ciencias Biológicas Virus Integration Molecular Sequence Data Genome Viral Microbiology Viral Evolution Mycobacterium Ciencias Biológicas Evolution Molecular Viral Proteins Lysogenic cycle Virology TM4 purl.org/becyt/ford/1.6 [https] Biology Comparative genomics Evolutionary Biology Base Sequence lcsh:R Virion Bacteriology Molecular Sequence Annotation Sequence Analysis DNA Comparative Genomics biology.organism_classification Mutation Cluster K lcsh:Q DNA viruses Gene Deletion |
| Zdroj: | PLoS ONE CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET PLoS ONE, Vol 6, Iss 10, p e26750 (2011) |
| ISSN: | 1932-6203 |
| Popis: | Five newly isolated mycobacteriophages -Angelica, CrimD, Adephagia, Anaya, and Pixie - have similar genomic architectures to mycobacteriophage TM4, a previously characterized phage that is widely used in mycobacterial genetics. The nucleotide sequence similarities warrant grouping these into Cluster K, with subdivision into three subclusters: K1, K2, and K3. Although the overall genome architectures of these phages are similar, TM4 appears to have lost at least two segments of its genome, a central region containing the integration apparatus, and a segment at the right end. This suggests that TM4 is a recent derivative of a temperate parent, resolving a long-standing conundrum about its biology, in that it was reportedly recovered from a lysogenic strain of Mycobacterium avium, but it is not capable of forming lysogens in any mycobacterial host. Like TM4, all of the Cluster K phages infect both fast- and slow-growing mycobacteria, and all of them - with the exception of TM4 - form stable lysogens in both Mycobacterium smegmatis and Mycobacterium tuberculosis; immunity assays show that all five of these phages share the same immune specificity. TM4 infects these lysogens suggesting that it was either derived from a heteroimmune temperate parent or that it has acquired a virulent phenotype. We have also characterized a widely-used conditionally replicating derivative of TM4 and identified mutations conferring the temperature-sensitive phenotype. All of the Cluster K phages contain a series of well conserved 13 bp repeats associated with the translation initiation sites of a subset of the genes; approximately one half of these contain an additional sequence feature composed of imperfectly conserved 17 bp inverted repeats separated by a variable spacer. The K1 phages integrate into the host tmRNA and the Cluster K phages represent potential new tools for the genetics of M. tuberculosis and related species. © 2011 Pope et al. Fil: Pope, Welkin H.. University of Pittsburgh; Estados Unidos Fil: Ferreira, Christina M.. University of Pittsburgh; Estados Unidos Fil: Jacobs Sera, Deborah. University of Pittsburgh; Estados Unidos Fil: Benjamin, Robert C.. University of North Texas; Estados Unidos Fil: Davis, Ariangela J.. Calvin College; Estados Unidos Fil: DeJong, Randall J.. Calvin College; Estados Unidos Fil: Elgin, Sarah C. R.. Washington University in St. Louis; Estados Unidos Fil: Guilfoile, Forrest R.. University of Pittsburgh; Estados Unidos Fil: Forsyth, Mark H.. The College Of William And Mary; Estados Unidos Fil: Harris, Alexander D.. Calvin College; Estados Unidos Fil: Harvey, Samuel E.. The College Of William And Mary; Estados Unidos Fil: Hughes, Lee E.. University of North Texas; Estados Unidos Fil: Hynes, Peter M.. Washington University in St. Louis; Estados Unidos Fil: Jackson, Arrykka S.. The College Of William And Mary; Estados Unidos Fil: Jalal, Marilyn D.. University of North Texas; Estados Unidos Fil: MacMurray, Elizabeth A.. The College Of William And Mary; Estados Unidos Fil: Manley, Coreen M.. University of North Texas; Estados Unidos Fil: McDonough, Molly J.. The College Of William And Mary; Estados Unidos Fil: Mosier, Jordan L.. University of North Texas; Estados Unidos Fil: Osterbann, Larissa J.. Calvin College; Estados Unidos Fil: Rabinowitz, Hannah S.. Washington University in St. Louis; Estados Unidos Fil: Rhyan, Corwin N.. Washington University in St. Louis; Estados Unidos Fil: Russell, Daniel A.. University of Pittsburgh; Estados Unidos Fil: Saha, Margaret S.. The College Of William And Mary; Estados Unidos Fil: Shaffer, Christopher D.. Washington University in St. Louis; Estados Unidos Fil: Simon, Stephanie E.. University of North Texas; Estados Unidos Fil: Sims, Erika F.. Washington University in St. Louis; Estados Unidos Fil: Tovar, Isabel G.. University of North Texas; Estados Unidos Fil: Weisser, Emilie G.. Washington University in St. Louis; Estados Unidos Fil: Wertz, John T.. Calvin College; Estados Unidos Fil: Weston-Hafer, Kathleen A.. Washington University in St. Louis; Estados Unidos Fil: Williamson, Kurt E.. The College Of William And Mary; Estados Unidos Fil: Zhang, Bo. Washington University in St. Louis; Estados Unidos Fil: Cresawn, Steven G.. James Madison University; Estados Unidos Fil: Jain, Paras. Albert Einstein College Of Medicine Of Yeshiva University; Estados Unidos Fil: Piuri, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University of Pittsburgh; Estados Unidos Fil: Jacobs, William R.. Albert Einstein College Of Medicine Of Yeshiva University; Estados Unidos Fil: Hendrix, Roger W.. University of Pittsburgh; Estados Unidos Fil: Hatfull, Graham F.. University of Pittsburgh; Estados Unidos |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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