Stable [57Co]-bleomycin complex with a very high specific radioactivity for use at very low concentrations
| Autor: | Lluis M. Mir, Jean Belehradek, B. Poddevin |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
congenital
hereditary and neonatal diseases and abnormalities Cell Survival Size-exclusion chromatography Biophysics chemistry.chemical_element Bleomycin Biochemistry Cell Line chemistry.chemical_compound Animals Bioassay Chelation Cobalt Radioisotopes Molecular Biology Volume concentration Isotope urogenital system nutritional and metabolic diseases Cobalt Cell Biology Electric Stimulation Clone Cells Kinetics chemistry Chromatography Gel Isotopes of cobalt Nuclear chemistry |
| Zdroj: | Biochemical and Biophysical Research Communications. 173:259-264 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/s0006-291x(05)81050-2 |
| Popis: | A method for the preparation of 57 Co-labelled bleomycin (BLM) possessing very high specific radioactivity and suitable for use at the nanomolar concentration range is described, and validated using a biological assay. Chelation of BLM with Co(II) results in a very stable complex. However, association does not occur below the micromolar concentration range. A nanomolar [ 57 Co]BLM solution with maximal specific radioactivity can be easily prepared, without handling unreasonable amounts of radioactivity, provided that : equimolar solutions of BLM and [ 57 Co]Cl 2 are first mixed at the micromolar concentration range and that the mixture is then diluted a thousand times to reach the nanomolar concentration range. |
| Databáze: | OpenAIRE |
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