Comparison of radioimmunoassay and chemiluminescent assay methods to estimate canine blood cortisol concentrations
| Autor: | P. Clark, S F Foster, Ian D. Robertson, Peter J. Irwin, D. Lewis, Nicholas J. Russell |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
medicine.medical_specialty
Adrenocortical Hyperfunction Time Factors Hydrocortisone Radioimmunoassay Sensitivity and Specificity law.invention Dogs Adrenocorticotropic Hormone Reference Values law Internal medicine medicine Animals Adrenal function Dog Diseases Chemiluminescence Chemiluminescence assay Blood Specimen Collection Dose-Response Relationship Drug General Veterinary Chemistry Significant difference Reproducibility of Results General Medicine Serum samples Dose–response relationship Endocrinology Blood Preservation Luminescent Measurements Validity data |
| Zdroj: | Australian Veterinary Journal. 85:487-494 |
| ISSN: | 1751-0813 0005-0423 |
| DOI: | 10.1111/j.1751-0813.2007.00232.x |
| Popis: | Non-radioactive assay methods are widely used in commercial laboratories to measure canine blood cortisol concentrations, despite a paucity of published validity data of these tests compared with the traditional 'gold standard' radioimmunoassay.To compare a commercial chemiluminescence assay with radioimmunoassay for blood cortisol measurement, determine the effect of storage on the radioimmunoassay, and determine the impact of any differences on clinical decisions.The study included 54 client owned dogs undergoing adrenal function testing. Fresh plasma or serum samples (n=170) were assayed for cortisol using radioimmunoassay (RIA1). Samples (n=196) were also frozen and stored in batches, and assayed by chemiluminescence and radioimmunoassay (RIA2).Overall, there was a strong correlation (r2=0.967, P0.001) between RIA2 and chemiluminescence concentrations without significant difference between means. Strong correlations were present for RIA2 and chemiluminescence at concentration subgroups of400 nmol/L (r2=0.869, P0.001),100 nmol/L (r2=0.790, P0.001), and40 nmol/L (r2=0.738, P0.001). Significant differences between means were present for RIA2 and chemiluminescence concentrations in the100 nmol/L, and40 nmol/L (P0.001) groups. Despite a significant difference in RIA1 and RIA2 results overall, there was no significant difference between RIA1 and RIA2 for any of the concentration groups. In seven cases, discrepant RIA2 and chemiluminescence results may have altered clinical decisions.Although RIA and chemiluminescence cortisol concentrations appear highly correlated, a significant difference may exist for concentrations less than 100 nmol/L in stored canine sera. Results of chemiluminescence cortisol assays should be interpreted with caution unless the specific assay method in the laboratory has been adequately validated in dogs. |
| Databáze: | OpenAIRE |
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