Regulation of Integrin-Mediated T Cell Adhesion by the Transmembrane Protein Tyrosine Phosphatase CD45
| Autor: | H, Shenoi, J, Seavitt, A, Zheleznyak, M L, Thomas, E J, Brown |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Hybridomas
T-Lymphocytes Immunology Intracellular Signaling Peptides and Proteins Membrane Proteins Phosphoproteins Peptide Fragments Enzyme Activation Jurkat Cells Mice Receptors Fibronectin Cell Adhesion Animals Humans Leukocyte Common Antigens Immunology and Allergy Protein Tyrosine Phosphatases Extracellular Space |
| Zdroj: | The Journal of Immunology. 162:7120-7127 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.162.12.7120 |
| Popis: | The transmembrane protein tyrosine phosphatase CD45 is required for Ag receptor signal transduction in lymphocytes. Recently, a role for CD45 in the regulation of macrophage adhesion has been demonstrated as well. To investigate further the role of CD45 in the regulation of adhesion, we examined integrin-mediated adhesion to fibronectin of two T cell lines and their CD45-deficient variants. The absence of CD45 correlated with enhanced adhesion to fibronectin via integrin α5β1 (VLA-5), but not α4β1 (VLA-4) in both cell lines. Adhesion returned to normal levels upon transfection of wild-type CD45 into the CD45-deficient lines. Transfection of chimeric or mutant molecules expressing some, but not all, CD45 domains and activities demonstrated that both the transmembrane domain and the tyrosine phosphatase activity of CD45 were required for regulation of integrin-dependent adhesion, but the highly glycosylated extracellular domain was dispensable. In contrast, only a catalytically active CD45 cytoplasmic domain was required for TCR signaling. Transfectants that restored normal levels of adhesion to fibronectin coimmunoprecipitated with the transmembrane protein known as CD45-associated protein. These studies demonstrate a novel role for CD45 in adhesion regulation and suggest a possible function for its association with CD45-associated protein. |
| Databáze: | OpenAIRE |
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