In vivo depth-resolved oxygen saturation by dual-wavelength photothermal (DWP) OCT

Autor: Roman V. Kuranov, Andrew K. Dunn, Jeffrey W. Kiel, S. M. Shams Kazmi, Thomas E. Milner, Austin McElroy, Timothy Q. Duong
Rok vydání: 2011
Předmět:
Male
Materials science
genetic structures
02 engineering and technology
Sensitivity and Specificity
01 natural sciences
010309 optics
Mice
symbols.namesake
Optics
ocis:(170.6510) Spectroscopy
tissue diagnostics
Optical coherence tomography
In vivo
Arteriole
medicine.artery
0103 physical sciences
ocis:(170.4500) Optical coherence tomography
medicine
Animals
Fiber Optic Technology
Oximetry
Oxygen saturation (medicine)
ocis:(300.1030) Absorption
medicine.diagnostic_test
business.industry
ocis:(120.5050) Phase measurement
Reproducibility of Results
Magnetic resonance imaging
Equipment Design
Photothermal therapy
021001 nanoscience & nanotechnology
Atomic and Molecular Physics
and Optics
respiratory tract diseases
Intensity (physics)
Equipment Failure Analysis
Oxygen
Arterioles
Refractometry
ocis:(170.1470) Blood or tissue constituent monitoring
symbols
Research-Article
sense organs
0210 nano-technology
business
Doppler effect
Tomography
Optical Coherence
circulatory and respiratory physiology
Zdroj: Optics Express
ISSN: 1094-4087
DOI: 10.1364/oe.19.023831
Popis: Microvasculature hemoglobin oxygen saturation (SaO2) is important in the progression of various pathologies. Non-invasive depth-resolved measurement of SaO2 levels in tissue microvasculature has the potential to provide early biomarkers and a better understanding of the pathophysiological processes allowing improved diagnostics and prediction of disease progression. We report proof-of-concept in vivo depth-resolved measurement of SaO(2) levels in selected 30 µm diameter arterioles in the murine brain using Dual-Wavelength Photothermal (DWP) Optical Coherence Tomography (OCT) with 800 nm and 770 nm photothermal excitation wavelengths. Depth location of back-reflected light from a target arteriole was confirmed using Doppler and speckle contrast OCT images. SaO(2) measured in a murine arteriole with DWP-OCT is linearly correlated (R(2)=0.98) with systemic SaO(2) values recorded by a pulse-oximeter. DWP-OCT are steadily lower (10.1%) than systemic SaO(2) values except during pure oxygen breathing. DWP-OCT is insensitive to OCT intensity variations and is a candidate approach for in vivo depth-resolved quantitative imaging of microvascular SaO(2) levels.
Databáze: OpenAIRE
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