Human β-Defensin 2 in Primary Sclerosing Cholangitis
| Autor: | Anchasa Kananurak, Christopher L. Bowlus, Cindy H. Chang, Ana Lleo, Pietro Invernizzi, Fabio Grizzi, Charles L. Bevins, Koichi Tsuneyama |
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| Přispěvatelé: | Chang, C, Lleo, A, Kananurak, A, Grizzi, F, Tsuneyama, K, Invernizzi, P, Bevins, C, Bowlus, C |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Original Contributions Chronic Liver Disease and Cirrhosis Clinical Sciences Autoimmune Disease digestive system Inflammatory bowel disease Oral and gastrointestinal Primary sclerosing cholangitis 03 medical and health sciences 0302 clinical medicine Clinical Research medicine 2.1 Biological and endogenous factors Copy-number variation Aetiology Gene Innate immune system business.industry Liver Disease Inflammatory Bowel Disease digestive oral and skin physiology Gastroenterology Primary sclerosing cholangitis (PSC) inflammatory bowel disease (IBD) human β-defensin 2 (HBD2) medicine.disease Ulcerative colitis digestive system diseases 3. Good health 030104 developmental biology Biliary ducts β defensin 2 Immunology 030211 gastroenterology & hepatology Digestive Diseases business |
| Zdroj: | Clinical and Translational Gastroenterology Clinical and translational gastroenterology, vol 8, iss 3 Chang, Cindy; Lleo, Ana; Kananurak, Anchasa; Grizzi, Fabio; Tsuneyama, Koichi; Invernizzi, Pietro; et al.(2017). Human β-Defensin 2 in Primary Sclerosing Cholangitis.. Clinical and translational gastroenterology, 8(3), e80. doi: 10.1038/ctg.2017.8. UC Davis: Retrieved from: http://www.escholarship.org/uc/item/14q3k6h6 |
| DOI: | 10.1038/ctg.2017.8. |
| Popis: | Author(s): Chang, Cindy; Lleo, Ana; Kananurak, Anchasa; Grizzi, Fabio; Tsuneyama, Koichi; Invernizzi, Pietro; Bevins, Charles L; Bowlus, Christopher L | Abstract: ObjectivesPrimary sclerosing cholangitis (PSC) is a chronic inflammatory disease of the bile ducts frequently associated with inflammatory bowel disease (IBD), suggesting an important role for the gut-liver axis. Defensins are small (3.5-4.5 kDa) anti-microbial peptides that contribute to innate immunity at mucosal surfaces and have been implicated in IBD. The aim of this study was to investigate copy number variation of the gene (DEFB4) encoding human β-defensin 2 (HBD2) and protein expression of HBD2 in PSC.MethodsUS and Italian PSC cases and unaffected controls (US PSC patients n=89, US controls n=87; Italian PSC patients n=46, Italian controls n=84) were used to estimate HBD2 gene copy number by both quantitative real-time PCR and paralog ratio test. Serum levels of HBD2 were measured by enzyme-linked immunosorbent assay and liver expression was analyzed by immunohistochemistry.ResultsMean serum levels of HBD2 were significantly greater in PSC (1,086±1,721 ng/μl) compared with primary biliary cholangitis (544±754 ng/μl), ulcerative colitis (417±506 ng/μl), and healthy controls (514±731 ng/μl) (P=0.02). However, no significant differences between the frequencies of high DEFB4 gene copy number, defined by g4 copies, and PSC were found in the US, Italian, or combined cohorts. Importantly, a high number of biliary ducts were found immunopositive in PSC samples compared with controls.ConclusionsOur data show that HBD2 serum levels and tissue expression are increased in PSC subjects, suggesting that this arm of innate immunity may be important in the etiopathogenesis of PSC. |
| Databáze: | OpenAIRE |
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