First case of Aspergillus caelatus airway colonization in a Chronic Obstructive Pulmonary Disease patient
| Autor: | Beatriz Ariza, Claudia Sierra, Rafael Matos Ximenes, Anita Montañez, Estelle Robert, Juan Iriarte, Patrice Le Pape, Carlos Alvarez-Moreno, Jaime Alvarado |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Antifungal Agents 030106 microbiology Virulence Microbial Sensitivity Tests Aspergillosis Microbiology lcsh:Infectious and parasitic diseases 03 medical and health sciences Pulmonary Disease Chronic Obstructive 0302 clinical medicine medicine Humans Colonization lcsh:RC109-216 030212 general & internal medicine Gene Aged Aspergillus COPD biology Sputum General Medicine biology.organism_classification medicine.disease Galleria mellonella Infectious Diseases Cross-Sectional Studies Dyspnea Treatment Outcome Female medicine.symptom |
| Zdroj: | International Journal of Infectious Diseases, Vol 81, Iss, Pp 85-90 (2019) |
| ISSN: | 1201-9712 |
| Popis: | Background: During a cross-sectional study on allergic aspergillosis in Chronic Obstructive Pulmonary Disease patients in Bogotá, Colombia, we reported the case of a 65-year-female patient with GOLD 2011 D classification, presenting dyspnea at the time of visit and aspergillus in repeated sputum cultures. Methods: The isolate was identified at the section level based on macroscopic and microscopic characteristics and gene sequencing was used for precise molecular identification. Antifungal sensibility was determined by Sensititre YeastOne™ while virulence was assessed using a Galleria mellonella larvae model. Results: The clinical isolate was first identified as Aspergillus section Flavi and sequencing of β-tubulin and calmodulin genes, in addition to the identification of alfR (aflatoxin regulator) gene, allowed the undoubted identification of the clinical isolate as Aspergillus caelatus. It exhibited virulence in G. mellonella similar to A. flavus and a high in vitro susceptibility against all antifungals except for amphotericin B. Conclusion: This is the first human case of airway colonization attributed to A. caelatus. Resistance pattern justified the interest to discriminate this cryptic species. Keywords: Aspergillus caelatus, COPD, Colonization, Aspergillosis |
| Databáze: | OpenAIRE |
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