Acid Extrusion from Human Spermatozoa Is Mediated by Flagellar Voltage-Gated Proton Channel
| Autor: | Polina V. Lishko, Yuriy Kirichok, Inna L. Botchkina, Andriy Fedorenko |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Male
endocrine system Voltage-gated proton channel Acrosome reaction HUMDISEASE DEVBIO Biology General Biochemistry Genetics and Molecular Biology Ion Channels Mice Extracellular Animals Humans Patch clamp reproductive and urinary physiology Sperm motility Sperm flagellum urogenital system Biochemistry Genetics and Molecular Biology(all) Acrosome Reaction Depolarization Hydrogen-Ion Concentration Sperm Spermatozoa Biochemistry SIGNALING Sperm Tail Biophysics Sperm Motility Female Sperm Capacitation |
| Zdroj: | Cell. 140(3):327-337 |
| ISSN: | 0092-8674 |
| DOI: | 10.1016/j.cell.2009.12.053 |
| Popis: | SummaryHuman spermatozoa are quiescent in the male reproductive system and must undergo activation once introduced into the female reproductive tract. This process is known to require alkalinization of sperm cytoplasm, but the mechanism responsible for transmembrane proton extrusion has remained unknown because of the inability to measure membrane conductance in human sperm. Here, by successfully patch clamping human spermatozoa, we show that proton channel Hv1 is their dominant proton conductance. Hv1 is confined to the principal piece of the sperm flagellum, where it is expressed at unusually high density. Robust flagellar Hv1-dependent proton conductance is activated by membrane depolarization, an alkaline extracellular environment, endocannabinoid anandamide, and removal of extracellular zinc, a potent Hv1 blocker. Hv1 allows only outward transport of protons and is therefore dedicated to inducing intracellular alkalinization and activating spermatozoa. The importance of Hv1 for sperm activation makes it an attractive target for controlling male fertility.PaperFlick |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|