Gene expression in juvenile arthritis and spondyloarthropathy: pro-angiogenic ELR+ chemokine genes relate to course of arthritis
| Autor: | David N. Glass, Michael G. Barnes, Robert A. Colbert, Edward H. Giannini, Raphael Hirsch, Marta B. Moroldo, Lorie Luyrink, Paul Pavlidis, Bruce J. Aronow, Susan D. Thompson, Murray H. Passo, Alexei A. Grom |
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| Rok vydání: | 2004 |
| Předmět: |
Adult
Chemokine Microarray Adolescent Spondyloarthropathy Arthritis Gene Expression Pilot Projects Biology Peripheral blood mononuclear cell Rheumatology Gene expression Synovial Fluid medicine Humans Pharmacology (medical) Child Gene Cells Cultured Oligonucleotide Array Sequence Analysis Retrospective Studies Neovascularization Pathologic Microarray analysis techniques Gene Expression Profiling Protein-Tyrosine Kinases medicine.disease Arthritis Juvenile Immunology biology.protein Leukocytes Mononuclear Trans-Activators Spondylarthropathies Chemokines CXC Signal Transduction |
| Zdroj: | Rheumatology (Oxford, England). 43(8) |
| ISSN: | 1462-0324 |
| Popis: | Objective. To evaluate the ability of microarray-based methods to identify genes with disease-specific expression patterns in peripheral blood mononuclear cells (PBMC) and synovial fluid mononuclear cells (SFMC) of juvenile arthritis patients and healthy controls. Methods. Microarray data (Affymetrix U95Av2) from 26 PBMC and 20 SFMC samples collected from patients with active disease (classified by course according to ACR criteria) were analysed for expression patterns that correlated with disease characteristics. For comparison, PBMC gene expression profiles were obtained from 15 healthy controls. Real-time PCR was used for confirmation of gene expression differences. Results. Statistical analysis of gene expression patterns in PBMC identified 378 probe sets corresponding to 342 unique genes with differing expression levels between polyarticular course patients and controls (t test, P < 0.0001). The genes represented by these probe sets were enriched for functions related to regulation of immune cell functions, receptor signalling as well as protein metabolism and degradation. Included in these probe sets were a group of CXCL chemokines with functions related to angiogenesis. Further analysis showed that, whereas angiogenic CXCL (ELR + ) gene expression was elevated in polyarticular PBMC, expression of angiostatic CXCL (ELR - ) chemokines was lower in polyarticular SFMC compared with corresponding pauciarticular samples (t test, P |
| Databáze: | OpenAIRE |
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