ELISA ANTI-HLA ANTIBODY SCREENING IDENTIFIES NON-COMPLEMENT-FIXING ANTIBODIES RESPONSIBLE FOR ACUTE GRAFT REJECTION. A CASE REPORT
| Autor: | A. Vangelista, Maria Scolari, G. Liviano D’Arcangelo, L. B. De Sanctis, Vittorio Bonomini, Andrea Buscaroli, Sergio Stefoni, R. Buttazzi, Paola Todeschini, S. Iannelli, A. Nanni-Costa |
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| Rok vydání: | 1996 |
| Předmět: |
Adult
Graft Rejection Male Urinary system Immunology Enzyme-Linked Immunosorbent Assay Human leukocyte antigen Flow cytometry HLA-B7 Antigen Immunopathology Genetics Humans Medicine Clinical significance Molecular Biology Genetics (clinical) Retrospective Studies Kidney medicine.diagnostic_test biology business.industry Complement Fixation Tests General Medicine Kidney Transplantation Transplantation medicine.anatomical_structure Immunoglobulin G biology.protein Antibody business |
| Zdroj: | Europe PubMed Central |
| ISSN: | 1744-3121 |
| DOI: | 10.1111/j.1744-313x.1996.tb00011.x |
| Popis: | We report on a kidney transplant recipient experiencing an unexpected early acute vascular graft rejection. Retrospective analysis of patient serum samples, utilizing a new ELISA HLA screening technique, revealed that the rejection crisis and the subsequent graft loss were due to a pretransplant donor-specific pre-sensitization caused by a non-complement-fixing antibody of IgG2 class. The case illustrates the clinical significance of non-complement-fixing anti-HLA antibodies. In addition it is shown that ELISA methods are suitable for detecting potentially harmful donor pre-sensitization in waiting-list patients not detectable by standard lymphocytotoxicity techniques. Hence ELISA could be an alternative to flow cytometry for this purpose. It is concluded that screening and cross-matching techniques which detect non-complement-fixing anti-HLA antibodies could improve graft outcome, and should form part of the immunological monitoring of kidney transplant waiting-list patients. |
| Databáze: | OpenAIRE |
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