Molecular characterization and antifungal susceptibilitytesting of Candida nivariensis from blood samples – an Iranian multicentre study and a review of the literature
| Autor: | Sadegh Khodavaisy, Carlo Brouwer, Farnaz Daneshnia, Amir Arastehfar, Teun Boekhout, Mohammad-Javad Najafzadeh, Kamiar Zomorodian, Maryam Roudbary, Ferry Hagen, Pieter-Jan A. Haas, Hossein Zarrinfar, Arezoo Charsizadeh, Mohammadreza Salehi, Weihua Pan |
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| Přispěvatelé: | Evolutionary and Population Biology (IBED, FNWI) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Antifungal Agents Iran Bronchoalveolar Lavage Polymerase Chain Reaction chemistry.chemical_compound Fatal Outcome Caspofungin Amplified Fragment Length Polymorphism Analysis Fluconazole Fluconazole/pharmacology Candida Vagina/microbiology Voriconazole/pharmacology Broth microdilution Candidiasis General Medicine Middle Aged 3. Good health Vagina Antifungal Agents/pharmacology DNA Intergenic Female medicine.drug Microbiology (medical) Adolescent Genotype 030106 microbiology Candidemia/diagnosis Microbial Sensitivity Tests Biology Microbiology 03 medical and health sciences Amphotericin B/pharmacology Candida/drug effects Caspofungin/pharmacology Amphotericin B medicine Humans Matrix-Assisted Laser Desorption-Ionization Genotyping Aged Voriconazole Intergenic Candida glabrata Spectrometry Micafungin Candidemia DNA Mass biology.organism_classification bacterial infections and mycoses Candidiasis/blood 030104 developmental biology chemistry Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Anidulafungin |
| Zdroj: | Journal of Medical Microbiology, 68(5), 770-777. Society for General Microbiology Journal of Medical Microbiology |
| ISSN: | 0022-2615 |
| Popis: | Purpose. Identification of the emerging yeast species Candida nivariensis among presumptively identified Iranian Candida glabrata isolates. Methodology. Clinical C. glabrata species complex isolates from blood (n=100; 46.9%), vaginal swabs (n=20; 9.4%), bronchoalveolar lavage (n=10; 4.7%) and sputum (n=12; 5.6%) from 68 patients from Iran were investigated. Isolates were characterized by CHROMagar, multiplex PCRs, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), amplified fragment length polymorphism (AFLP) fingerprinting, internal transcribed spacer (ITS)/large subunit (LSU) rDNA and FKS1/FKS2 sequencing, and the European Committee on Antimicrobial Susceptibility Testing broth microdilution method. A comprehensive literature review was conducted and all the relevant clinical and microbiological data were collected.Results. Four C. nivariensis isolates were recovered from blood samples of three subjects and were all consistently identified by nine-plex PCR, Bruker MALDI-TOF MS, and LSU and ITS rDNA sequencing. AFLP genotyping clustered the isolates into two groups. Sequencing of the FKS1 and FKS2 hotspots showed no accountable amino acid substitutions. All isolates were susceptible to amphotericin B, fluconazole, itraconazole, posaconazole, voriconazole, anidulafungin and micafungin.Conclusion. In total, 4 out of 213 clinical C. glabrata species complex candidemia isolates were C. nivariensis. Improvement of the BioMerieux Vitek MS database is required to accurately identify C. nivariensis and it is advised to alternatively use CHROMagar and/or PCR-based techniques. As other species within the Nakaseomyces clade may cause infection and showed high MIC values for antifungals, inclusion of their spectra into the MALDI-TOF MS database seems relevant. Due to developing resistance to fluconazole and insufficient efficacy of caspofungin, the combination of catheter removal plus treatment with caspofungin, or voriconazole, or micafungin might be effective for patients. |
| Databáze: | OpenAIRE |
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