Mitochondrial Complex I Activity is Conditioned by Supercomplex I–III2–IV Assembly in Brain Cells: Relevance for Parkinson’s Disease
| Autor: | Angeles Almeida, Monica Carabias-Carrasco, Juan P. Bolaños, Monica Resch-Beusher, Irene Lopez-Fabuel |
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| Přispěvatelé: | Ministerio de Economía y Competitividad (España), Instituto de Salud Carlos III, European Commission, Centro de Investigación Biomédica en Red de Fragilidad y Envejecimiento Saludable (España) |
| Jazyk: | angličtina |
| Předmět: |
0301 basic medicine
Parkinson's disease Rodent Bioenergetics Mitochondrion Biochemistry 03 medical and health sciences Cellular and Molecular Neuroscience Complexes biology.animal medicine Citrate synthase chemistry.chemical_classification Neurons Reactive oxygen species biology General Medicine medicine.disease Molecular biology 3. Good health Mitochondria 030104 developmental biology Mitochondrial respiratory chain chemistry Astrocytes biology.protein Parkinson’s disease Specific activity Neuroscience |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname Neurochemical Research |
| ISSN: | 1573-6903 0364-3190 |
| DOI: | 10.1007/s11064-017-2191-2 |
| Popis: | The assembly of complex I (CI) with complexes III (CIII) and IV (CIV) of the mitochondrial respiratory chain (MRC) to configure I–III- or I–III–IV-containing supercomplexes (SCs) regulates mitochondrial energy efficiency and reactive oxygen species (mROS) production. However, whether the occurrence of SCs impacts on CI specific activity remains unknown to our knowledge. To investigate this issue, here we determined CI activity in primary neurons and astrocytes, cultured under identical antioxidants-free medium, from two mouse strains (C57Bl/6 and CBA) and Wistar rat, i.e. three rodent species with or without the ability to assemble CIV into SCs. We found that CI activity was 6- or 1.8-fold higher in astrocytes than in neurons, respectively, from rat or CBA mouse, which can form I–III2–IV SC; however, CI activity was similar in the cells from C57Bl/6 mouse, which does not form I–III2–IV SC. Interestingly, CII–III activity, which was comparable in neurons and astrocytes from mice, was about 50% lower in astrocytes when compared with neurons from rat, a difference that was abolished by antioxidants- or serum-containing media. CIV and citrate synthase activities were similar under all conditions studied. Interestingly, in rat astrocytes, CI abundance in I–III2–IV SC was negligible when compared with its abundance in I–III-containing SCs. Thus, CIV-containing SCs formation may determine CI specific activity in astrocytes, which is important to understand the mechanism for CI deficiency observed in Parkinson’s disease. J.P.B. is funded by MINECO (SAF2013-41177-R, SAF2016-78114-R), CIBER on Frailty and Aging from the Instituto de Salud Carlos III (CB16/10/00282), E.U. SP3-People-MC-ITN programme (608381), EU BATCure Grant (666918) and FEDER (European regional development fund). A.A.P. is funded by the Instituto de Salud Carlos III (RD12/0014/0007). |
| Databáze: | OpenAIRE |
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