Evaluation of intrarenal oxygenation in iodinated contrast-induced acute kidney injury-susceptible rats by blood oxygen level-dependent magnetic resonance imaging
| Autor: | Ujala Bokhary, Anindya Sen, Pottumarthi V. Prasad, Richard Solomon, Ying Zhou, Jing Lu, Maria V. Papadopoulou, Lu-Ping Li, Tammy Franklin |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Pathology medicine.medical_specialty Iohexol Ioxaglic acid chemistry.chemical_element Contrast Media Oxygen Article Rats Sprague-Dawley Iodinated contrast Lipocalin-2 Proto-Oncogene Proteins Triiodobenzoic Acids medicine Ioxaglic Acid Animals Radiology Nuclear Medicine and imaging Blood-oxygen-level dependent medicine.diagnostic_test business.industry Acute kidney injury Magnetic resonance imaging General Medicine Oxygenation Acute Kidney Injury medicine.disease Magnetic Resonance Imaging Iothalamic Acid Lipocalins chemistry Feasibility Studies business Biomarkers medicine.drug Acute-Phase Proteins |
| Zdroj: | Investigative radiology. 49(6) |
| ISSN: | 1536-0210 |
| Popis: | The objectives of this study were to evaluate differences in intrarenal oxygenation as assessed by blood oxygen level-dependent (BOLD) magnetic resonance imaging in contrast-induced acute kidney injury (CIAKI)-susceptible rats when using 4 contrast media with different physicochemical properties and to demonstrate the feasibility of acquiring urinary neutrophil gelatinase-associated lipocalin (NGAL) levels as a marker of CIAKI in this model.Our institutional animal care and use committee approved the study. Sixty-six Sprague-Dawley rats were divided into CIAKI-susceptible groups (received nitric oxide synthase inhibitor N-nitro-L-arginine methyl ester [10 mg/kg] and cycloxygenase inhibitor indomethacin [10mg/kg]) and control groups (received saline instead). One of the 4 iodinated contrast agents (iothalamate, iohexol, ioxaglate, or iodixanol) was then administered (1600-mg organic iodine per kilogram of body weight). Multiple blood oxygen level-dependent magnetic resonance images were acquired on a Siemens 3.0-T scanner using a multiple gradient recalled echo sequence at baseline, after N-nitro-L-arginine methyl ester (or saline), indomethacin (or saline), and iodinated contrast agent (or placebo). R2* (R2*=1/T2*) maps were generated inline on the scanner. A mixed-effects growth curve model with first-order autoregressive variance-covariance was used to analyze the temporal data. Urinary NGAL, a marker of kidney injury (unlike serum creatinine), was measured 4 hours after contrast injection in the 2 subgroups.Differences in blood oxygen level-dependent magnetic resonance imaging results between the contrast media were observed in all 4 renal regions. However, the inner stripe of the outer medulla (ISOM) showed the most pronounced changes in the CIAKI-susceptible group and R2* increased significantly (P0.01) over time with all 4 contrast media. In the control groups, only iodixanol showed an increase in R2* (P0.05) over time. There was an agreement between increases in NGAL and R2* values in ISOM.In rats susceptible to CIAKI, those receiving contrast media had significant increases in R2* in renal ISOM compared with those receiving placebo. The agreement between NGAL and R2* values in the ISOM suggests that the observed immediate increase in R2* after contrast injection may be the earliest biomarker of renal injury. Further studies are necessary to establish threshold values of R2* associated with acute kidney injury and address the specificity of R2* to renal oxygenation status. |
| Databáze: | OpenAIRE |
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