Diagnostic utility of CD4%:CD8low% T-lymphocyte ratio to differentiate feline immunodeficiency virus (FIV)-infected from FIV-vaccinated cats
| Autor: | Jamieson Nichols, Annette Litster, Jui-Ming Lin, Hsin-Yi Weng |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
CD4-Positive T-Lymphocytes
Male Feline immunodeficiency virus animal diseases viruses CD4-CD8 Ratio Enzyme-Linked Immunosorbent Assay CD8-Positive T-Lymphocytes Immunodeficiency Virus Feline Microbiology Western blot Feline Acquired Immunodeficiency Syndrome medicine Animals CATS General Veterinary biology medicine.diagnostic_test Vaccination virus diseases General Medicine T lymphocyte biochemical phenomena metabolism and nutrition Feline immunodeficiency virus FIV biology.organism_classification Virology Immunology Cats biology.protein Female Antibody CD8 |
| Zdroj: | Veterinary Microbiology. 170:197-205 |
| ISSN: | 0378-1135 |
| DOI: | 10.1016/j.vetmic.2014.01.014 |
| Popis: | Antibody testing based on individual risk assessments is recommended to determine feline immunodeficiency virus (FIV) status, but ELISA and Western blot tests cannot distinguish between anti-FIV antibodies produced in response to natural infection and those produced in response to FIV vaccination. The aim of this cross-sectional study was to test the hypothesis that FIV-infected cats could be differentiated from FIV-vaccinated uninfected cats using lymphocyte subset results, specifically the CD4%:CD8low% T-lymphocyte ratio. Comparisons of the CD4%:CD8low% T-lymphocyte ratio were made among the following four groups: Group 1 – FIV-infected cats (n = 61; FIV-antibody positive by ELISA and FIV PCR positive); Group 2 – FIV-uninfected cats (n = 96; FIV-antibody negative by ELISA); Group 3 – FIV-vaccinated uninfected cats (n = 31; FIV-antibody negative by ELISA before being vaccinated against FIV, after which they tested FIV ELISA positive); and Group 4 – FIV-uninfected but under chronic/active antigenic stimulation (n = 16; FIV-antibody negative by ELISA; all had active clinical signs of either upper respiratory tract disease or gingival disease for ≥ 21 days). The median CD4%:CD8low% T-lymphocyte ratio was lower in Group 1 (1.39) than in each of the other three groups (Group 2 – 9.77, Group 3 – 9.72, Group 4 – 5.64; P < 0.05). The CD4%:CD8low% T-lymphocyte ratio was also the most effective discriminator between FIV-infected cats and the other three groups, and areas under ROC curves ranged from 0.91 (compared with Group 4) to 0.96 (compared with Group 3). CD4%:CD8low% shows promise as an effective test to differentiate between FIV-infected cats and FIV-vaccinated uninfected cats. |
| Databáze: | OpenAIRE |
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