The effects of topical melatonin on oxidative stress, apoptosis signals, and p53 protein expression during cutaneous wound healing
| Autor: | Nazli Gül Altindiş, Halil Aksoy, Ozge Cevik, Özge Doğan, Betül Okuyan, Azize Şener |
|---|---|
| Přispěvatelé: | Sivas Cumhuriyet Üniversitesi, [Sener, Azize -- Dogan, Ozge -- Altindis, Nazli Gul -- Aksoy, Halil] Marmara Univ, Fac Pharm, Dept Biochem, Istanbul, Turkey -- [Cevik, Ozge] Cumhuriyet Univ, Fac Pharm, Dept Biochem, Sivas, Turkey -- [Okuyan, Betul] Marmara Univ, Fac Pharm, Dept Clin Pharm, Istanbul, Turkey, Cevik, Ozge -- 0000-0002-9325-3757 |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
p53
medicine.medical_specialty Antioxidant Physiology medicine.medical_treatment Apoptosis wound healing medicine.disease_cause Microbiology Melatonin chemistry.chemical_compound Hydroxyproline Internal medicine Genetics medicine oxidative stress Bcl-2 Molecular Biology Biology chemistry.chemical_classification Reactive oxygen species biology integumentary system Apoptosis Bcl-2 p53 oxidative stress topical melatonin wound healing topical melatonin Cell Biology Malondialdehyde Endocrinology chemistry Myeloperoxidase biology.protein General Agricultural and Biological Sciences Wound healing Oxidative stress hormones hormone substitutes and hormone antagonists Biyoloji medicine.drug |
| Zdroj: | Volume: 39, Issue: 6 888-895 Turkish Journal of Biology |
| ISSN: | 1300-0152 1303-6092 |
| Popis: | WOS: 000365505600011 Elimination of reactive oxygen species (ROS) can be an important strategy to improve healing of wounds. ROS have an effect on proliferation and cell survival signaling, which results in alteration of apoptotic pathways in cells. Melatonin has antioxidant properties on skin wounds. In our study, we investigated the effects of topical melatonin (3%, w/w) on apoptosis and p53 protein expression together with parameters of oxidative stress in a cutaneous excision wound model. Bcl-2 protein levels in wound tissue at the end of days 3, 7, and 14 were significantly increased, while caspase-3 activity and p53 protein expression in wound tissue at the end of days 3, 7, and 14 were also reduced with melatonin treatment during wound healing. On days 3 and 7 after the wound, malondialdehyde level was reduced and glutathione was increased with melatonin treatment. Melatonin decreased myeloperoxidase levels and increased hydroxyproline levels in wound tissue at the end of day 7. However, melatonin had no significant effect on percentage of wound closure. Considering our results, topical melatonin displays antioxidant, antiapoptotic, and p53-inhibitory effects, but these effects are not sufficient for the acceleration of wound closure. Marmara University Research Unit [SAG-D-050614-0237] This work was supported by the Marmara University Research Unit (Grant No: SAG-D-050614-0237). The authors would like to thank Prof Dr Goksel Sener for her contribution. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|