Characterisation of an intracellular Ca2+ pump in Dictyostelium
| Autor: | M. Satre, E.K. Rooney, J.D. Gross |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Thapsigargin
Physiology ATPase Protozoan Proteins chemistry.chemical_element Calcium-Transporting ATPases Calcium Dictyostelium discoideum Fungal Proteins chemistry.chemical_compound Microsomes Centrifugation Density Gradient Animals Dictyostelium Vanadate Molecular Biology Oxalates biology Terpenes Vesicle Intracellular Membranes Cell Biology biology.organism_classification Cell Compartmentation Contractile vacuole Proton-Translocating ATPases chemistry Biochemistry Nigericin Vacuoles biology.protein Protons Vanadates Intracellular |
| Zdroj: | Cell Calcium. 16:509-522 |
| ISSN: | 0143-4160 |
| DOI: | 10.1016/0143-4160(94)90081-7 |
| Popis: | Calcium uptake by microsomal membranes from the cellular slime mould Dictyostelium discoideum was measured using Calcium Green-2 as a fluorescent probe of external free Ca 2+ concentration. High-affinity Ca 2+ uptake was found to be completely inhibited by low concentrations of vanadate, but not by thapsigargin, suggesting that the activity is mediated by a Ca 2+ -ATPase distinct from sarco(endo)plasmic reticulum type of higher animal cells. On sucrose density gradients, Ca 2+ uptake distributes with vacuolar proton pump activity and part of the observed Ca 2+ uptake is dependent on the pH gradient generated by the vacuolar-type H + -ATPase, indicating that the Ca 2+ pump is located on both acidic and non-acidic vesicles, possibly derived from the H + -ATPase-rich contractile vacuole complex. |
| Databáze: | OpenAIRE |
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