Winter Aconite (Eranthis hyemalis) Lectin as a cytotoxic effector in the lifecycle of Caenorhabditis elegans
| Autor: | David R. Lisgarten, Simon C. Harvey, Emilia Bértolo, Lee J. Byrne, Marie T. McConnell |
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| Rok vydání: | 2015 |
| Předmět: |
lcsh:Medicine
Eranthis hyemalis N-acetyl-D-galactosamine Toxicology Biochemistry General Biochemistry Genetics and Molecular Biology Genetics Caenorhabditis elegans Dauer larvae chemistry.chemical_classification biology Effector General Neuroscience Ribosome-inactivating protein lcsh:R fungi Winter Aconite Lectin Lectin Amphid General Medicine biology.organism_classification Ribosome Inactivating Protein Phenotype Amino acid Cell biology chemistry biology.protein General Agricultural and Biological Sciences Developmental Biology |
| Zdroj: | PeerJ PeerJ, Vol 3, p e1206 (2015) |
| ISSN: | 2167-8359 |
| Popis: | The lectin found in the tubers of the Winter Aconite (Eranthis hyemalis) plant is an N-acetyl-D-galactosamine specific Type II Ribosome Inactivating Protein (RIP); Type II RIPs have shown anti-cancer properties, and hence have potential as therapeutic agents. Here we present a modified protocol for the extraction and purification of the E. hyemalis lectin (EHL) using affinity chromatography. De novo amino acid sequencing of EHL confirms its classification as a Type II Ribosome Inactivating Protein. The biocidal properties of EHL have been investigated against the nematode Caenorhabditis elegans. Arrested first stage larvae treated with EHL have shown some direct mortality, with surviving larvae subsequently showing a range of phenotypes including food avoidance, reduced fecundity, developmental delay and constitutive dauer larvae formation. Both inappropriate dauer larvae development and failure to locate to bacterial food source are consistent with the disruption of chemosensory function and the ablation of amphid neurons. Further investigation indicates that mutations that disrupt normal amphid formation can block the EHL-induced dauer larvae formation. In combination, these phenotypes indicate that EHL is cytotoxic and suggest a cell specific activity against the amphid neurons of C. elegans. |
| Databáze: | OpenAIRE |
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